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Goat IgG (Fc) fragment Rabbit mAb (A23074)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Goat

ABclonal:Western blot - Goat IgG (Fc) fragment Rabbit mAb (A23074)

Western blot analysis of recombinant Goat IgG (Fc) fragment and Goat IgG (Fab) fragment(Negative control) , using Goat IgG (Fc) fragment Rabbit mAb (A23074) at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 10ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product nameGoat IgG (Fc) fragment Rabbit mAb
Catalog No.A23074
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC56943
ImmunogenGoat IgG
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW62kDa
ReactivityGoat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:6000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesRecombinant Goat IgG (Fc) fragment
Cellular location
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Goat IgG (Fc) fragment Rabbit mAb (A23074)}

    Western blot - Goat IgG (Fc) fragment Rabbit mAb (A23074)

    Western blot analysis of recombinant Goat IgG (Fc) fragment and Goat IgG (Fab) fragment(Negative control) , using Goat IgG (Fc) fragment Rabbit mAb (A23074) at 1:5000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 10ng per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A23074? Please let us know so that we can cite the reference in this datasheet.