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HRP-conjugated Donkey anti-Rabbit IgG (H+L) (AS038)

Publications (17) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:

ABclonal:Western blot - HRP-conjugated Donkey anti-Rabbit IgG (H+L) (AS038)

Western blot analysis of lysates from HeLa cells, using β-actin antibody as the primary antibody.
Secondary antibody: HRP Donkey Anti-Rabbit IgG (H+L) antibody (AS038) at 1:2000-1:30000 dilution
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - HRP-conjugated Donkey anti-Rabbit IgG (H+L) (AS038)

Immunohistochemistry analysis of paraffin-embedded Human liver (primary antibody is acox1) using HRP Donkey Anti-Rabbit IgG (H+L) (AS038) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - HRP-conjugated Donkey anti-Rabbit IgG (H+L) (AS038)

Immunohistochemistry analysis of paraffin-embedded Human liver (the primary antibody is acox1, And the control secondary antibody is stained) using HRP Donkey Anti-Rabbit IgG (H+L) (AS038) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product nameHRP-conjugated Donkey anti-Rabbit IgG (H+L)
Catalog No.AS038
Host speciesDonkey
Purification methodAffinity purification
IsotypeHorseradish peroxidase conjugated IgG
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
ImmunogenRabbit IgG
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW42kDa
Reactivity
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:5000 - 1:100000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.75% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa
Cellular location
Customer validation

WB(Mus musculus, Homo sapiens, Chlorocebus aethiops , Oryctolagus cuniculus, Rattus norvegicus, Capra hircus)

IHC(Mus musculus, Homo sapiens)

WB(Mus musculus)

IF(Oryza sativa)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - HRP-conjugated Donkey anti-Rabbit IgG (H+L) (AS038)}

    Western blot - HRP-conjugated Donkey anti-Rabbit IgG (H+L) (AS038)

    Western blot analysis of lysates from HeLa cells, using β-actin antibody as the primary antibody.
    Secondary antibody: HRP Donkey Anti-Rabbit IgG (H+L) antibody (AS038) at 1:2000-1:30000 dilution
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunohistochemistry - HRP-conjugated Donkey anti-Rabbit IgG (H+L) (AS038)}

    Immunohistochemistry - HRP-conjugated Donkey anti-Rabbit IgG (H+L) (AS038)

    Immunohistochemistry analysis of paraffin-embedded Human liver (primary antibody is acox1) using HRP Donkey Anti-Rabbit IgG (H+L) (AS038) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - HRP-conjugated Donkey anti-Rabbit IgG (H+L) (AS038)}

    Immunohistochemistry - HRP-conjugated Donkey anti-Rabbit IgG (H+L) (AS038)

    Immunohistochemistry analysis of paraffin-embedded Human liver (the primary antibody is acox1, And the control secondary antibody is stained) using HRP Donkey Anti-Rabbit IgG (H+L) (AS038) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AS038? Please let us know so that we can cite the reference in this datasheet.