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HRP-conjugated Goat anti-Human IgG (H+L) (AS002)

Publications (23) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:

ABclonal:HRP-conjugated Goat anti-Human IgG (H+L)
ABclonal:Western blot - HRP-conjugated Goat anti-Human IgG (H+L) (AS002)

Western blot analysis of lysates from Human IgG, using HRP Goat Anti-Human IgG (H+L) (AS002) at 1:2000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - HRP-conjugated Goat anti-Human IgG (H+L) (AS002)

Western blot analysis of lysates from Human IgG using HRP Goat Anti-Human IgG (H+L) (AS002) at 1:10000 dilution.
Lysates/proteins: 50ng - 100ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

Overview

Product nameHRP-conjugated Goat anti-Human IgG (H+L)
Catalog No.AS002
Host speciesGoat
Purification methodAffinity purification
IsotypeHorseradish peroxidase conjugated IgG
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
ImmunogenHuman IgG
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW25kDa, 55kDa
Reactivity
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:10000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.75% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHuman IgG1, Human IgG2, Human IgG4
Cellular location
Customer validation

ELISA(Homo sapiens, Mus musculus)

IHC(Homo sapiens)

WB(Mesocricetus auratus, Rattus norvegicus, Anatinae, Homo sapiens, Sus scrofa)

IF(Homo sapiens)

Documents

Certificate of Compliance

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    ABclonal:Western blot - HRP-conjugated Goat anti-Human IgG (H+L) (AS002)}

    Western blot - HRP-conjugated Goat anti-Human IgG (H+L) (AS002)

    Western blot analysis of lysates from Human IgG, using HRP Goat Anti-Human IgG (H+L) (AS002) at 1:2000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Western blot - HRP-conjugated Goat anti-Human IgG (H+L) (AS002)}

    Western blot - HRP-conjugated Goat anti-Human IgG (H+L) (AS002)

    Western blot analysis of lysates from Human IgG using HRP Goat Anti-Human IgG (H+L) (AS002) at 1:10000 dilution.
    Lysates/proteins: 50ng - 100ng per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 20s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AS002? Please let us know so that we can cite the reference in this datasheet.