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HRP-conjugated Goat anti-Mouse IgG Light Chain (AS062)

Publications (24) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Western blot - HRP-conjugated Goat anti-Mouse IgG Light Chain (AS062)

Western blot analysis of lysates from mouse IgG, using HRP-conjugated Goat Anti-Mouse IgG Light Chain (AS062) at 1:5000 dilution.
Lysates/proteins: 100ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

Overview

Product nameHRP-conjugated Goat anti-Mouse IgG Light Chain
Catalog No.AS062
Host speciesGoat
Purification methodAffinity purification
IsotypeIgG
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
ImmunogenMouse IgG
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples
Cellular location
Customer validation

WB(Rattus norvegicus, Anatinae, Sus scrofa, Mus musculus, Homo sapiens, Mus musculus, Other, Chlorocebus aethiops)

IP(Homo sapiens, Mus musculus)

Co-IP(Homo sapiens, Anatinae)

IF(Homo sapiens, Mus musculus)

WB(Anatinae)

Documents

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    ABclonal:Western blot - HRP-conjugated Goat anti-Mouse IgG Light Chain (AS062)}

    Western blot - HRP-conjugated Goat anti-Mouse IgG Light Chain (AS062)

    Western blot analysis of lysates from mouse IgG, using HRP-conjugated Goat Anti-Mouse IgG Light Chain (AS062) at 1:5000 dilution.
    Lysates/proteins: 100ng per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AS062? Please let us know so that we can cite the reference in this datasheet.