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HRP-conjugated Mouse anti GST-Tag mAb (AE027)

Publications (4) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Western blot - HRP-conjugated Mouse anti GST-Tag mAb (AE027)

Western blot analysis of recombinant GST Protein using HRP-conjugated Mouse anti GST-Tag mAb (AE027) at 1:5000 dilution.
Lysates/proteins: 20 ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product nameHRP-conjugated Mouse anti GST-Tag mAb
Catalog No.AE027
Host speciesMouse
Purification methodAffinity purification
Isotypeigg2b, Kappa
CloneNo.AMC0513
Glutathione S-transferases (GSTs), previously known as ligandins, comprise a family of eukaryotic and prokaryotic phase II metabolic isozymes best known for their ability to catalyze the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates for the purpose of detoxification. The GST family consists of three superfamilies: the cytosolic, mitochondrial, and microsomal—also known as MAPEG—proteins. Members of the GST superfamily are extremely diverse in amino acid sequence, and a large fraction of the sequences deposited in public databases are of unknown function. The Enzyme Function Initiative (EFI) is using GSTs as a model superfamily to identify new GST functions.A GST-tag is often used to separate and purify proteins that contain the GST-fusion protein. The tag is 220 amino acids (roughly 26 KDa) in size, which, compared to tags such as the Myc-tag or the FLAG-tag, is quite large. It can be fused to either the N-terminus or C-terminus of a protein. However, many commercially available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification.
ImmunogenRecombinant protein containing a sequence corresponding to amino acids 1-218 of GST protein.
SequenceMSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK
Gene ID
Swiss Prot
SynonymsGST; GST tag; GST-tag
Calculated MW26kDa
Observed MW27kDa
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:10000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesRecombinant GST Protein
Cellular location
Customer validation

WB(Homo sapiens, Escherichia coli, Glycine max)

Documents

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    ABclonal:Western blot - HRP-conjugated Mouse anti GST-Tag mAb (AE027)}

    Western blot - HRP-conjugated Mouse anti GST-Tag mAb (AE027)

    Western blot analysis of recombinant GST Protein using HRP-conjugated Mouse anti GST-Tag mAb (AE027) at 1:5000 dilution.
    Lysates/proteins: 20 ng per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AE027? Please let us know so that we can cite the reference in this datasheet.