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HRP-conjugated Mouse anti HA-Tag mAb (AE025)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Western blot - HRP-conjugated Mouse anti HA-Tag mAb (AE025)

Western blot analysis of extracts of normal 293T cells, 293T transfected with SCRN1 Protein , using HRP-conjugated Mouse anti HA-Tag mAb (AE025) at 1:5000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - HRP-conjugated Mouse anti HA-Tag mAb (AE025)

Western blot analysis of extracts of normal 293T and 293F cells, 293T transfected with CD2 Protein and HA-N Protein , 293F transfected with CD95-N Protein using HRP-conjugated Mouse anti HA-Tag mAb (AE025) at 1:5000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Western blot - HRP-conjugated Mouse anti HA-Tag mAb (AE025)

Western blot analysis of extracts of normal 293T and 293F cells, 293T transfected with NR3C2-C Protein, 293F transfected with WDR77-C Protein using HRP-conjugated Mouse anti HA-Tag mAb (AE025) at 1:5000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameHRP-conjugated Mouse anti HA-Tag mAb
Catalog No.AE025
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG1, Kappa
CloneNo.AMC0557
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
ImmunogenA synthetic peptide corresponding to HA tag.
SequenceYPYDVPDYA
Gene ID
Swiss Prot
SynonymsHA; HA tag; HA-tag
Calculated MW
Observed MW53kDa(CD95)/65kDa(CD2)/53kDa(SCRN1)/26KD(HA(N端)/42kDa(WDR77)/45kDa(NR3C2)
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:5000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T-CD2-3*HA(N), 293T-CD2-3*HA(C), 293F, 293F-CD95-3*HA(N), 293F-CD95-3*HA(C)293F-WDR77-HA-GFP(C), 293T-SCRN1-HA-GFP(N), 293T-SCRN1-HA-GFP(C), 293T-HA(N)
Cellular location
Customer validation

WB(Mus musculus)

IP(Mus musculus)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - HRP-conjugated Mouse anti HA-Tag mAb (AE025)}

    Western blot - HRP-conjugated Mouse anti HA-Tag mAb (AE025)

    Western blot analysis of extracts of normal 293T cells, 293T transfected with SCRN1 Protein , using HRP-conjugated Mouse anti HA-Tag mAb (AE025) at 1:5000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Western blot - HRP-conjugated Mouse anti HA-Tag mAb (AE025)}

    Western blot - HRP-conjugated Mouse anti HA-Tag mAb (AE025)

    Western blot analysis of extracts of normal 293T and 293F cells, 293T transfected with CD2 Protein and HA-N Protein , 293F transfected with CD95-N Protein using HRP-conjugated Mouse anti HA-Tag mAb (AE025) at 1:5000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 3s.
    ABclonal:Western blot - HRP-conjugated Mouse anti HA-Tag mAb (AE025)}

    Western blot - HRP-conjugated Mouse anti HA-Tag mAb (AE025)

    Western blot analysis of extracts of normal 293T and 293F cells, 293T transfected with NR3C2-C Protein, 293F transfected with WDR77-C Protein using HRP-conjugated Mouse anti HA-Tag mAb (AE025) at 1:5000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AE025? Please let us know so that we can cite the reference in this datasheet.