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HRP-conjugated Mouse anti His-Tag mAb (AE028)

Publications (26) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Western blot - HRP-conjugated Mouse anti His-Tag mAb (AE028)

Western blot analysis of extracts of normal 293T cells and 293T transfected with His-NAA20 protein, using HRP-conjugated Mouse anti His-Tag mAb (AE028) at 1:20000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product nameHRP-conjugated Mouse anti His-Tag mAb
Catalog No.AE028
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG2B, Kappa
CloneNo.AMC0484
Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected by using His antibodies.
ImmunogenA synthetic peptide corresponding to His tag.
SequenceHHHHHH
Gene ID
Swiss Prot
SynonymsHis; His tag; His-tag
Calculated MW
Observed MWRefer to Figures
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:10000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T
Cellular location
Customer validation

.(Oryza sativa)

WB(Homo sapiens, Mus musculus, Danio rerio, Triticum aestivum, Listeria monocytogenes, Staphylococcus aureus, Enterococcus faecalis, Arabidopsis thaliana, Escherichia coli, Glycine max, Other)

Other(Arabidopsis thaliana)

ELISA(Other)

Documents

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    ABclonal:Western blot - HRP-conjugated Mouse anti His-Tag mAb (AE028)}

    Western blot - HRP-conjugated Mouse anti His-Tag mAb (AE028)

    Western blot analysis of extracts of normal 293T cells and 293T transfected with His-NAA20 protein, using HRP-conjugated Mouse anti His-Tag mAb (AE028) at 1:20000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AE028? Please let us know so that we can cite the reference in this datasheet.