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HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb (AS122)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:

ABclonal: - HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb (AS122)

Dose response curve of HRP conjugated Mouse Anti-Rabbit IgG (Fc) mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate., blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Mouse Anti-Rabbit IgG (Fc) mAb in a 2 fold serial dilution from 2 μg/mL to 1.22*10^-4 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated thatMouse Anti-Rabbit IgG (Fc) mAb has highly specific recognition of Rabbit IgG while no or minimal cross reactivity to Rabbit IgM、 Human IgG、Mouse IgG、Rat IgG.

ABclonal:Western blot - HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb (AS122)

Western blot analysis of lysates from Rabbit IgG using HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb (AS122) at 1:1000-1:20000 dilution incubated overnight at 4℃.
Lysates/proteins: 100ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product nameHRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
Catalog No.AS122
Host speciesMouse
Purification methodAffinity purification
Isotypemouse IgG1
CloneNo.AMC50001-HRP
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 96-323 of rabbit IgG (Fc) (P01870).
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW55kDa
Observed MW55kDa//55kDa
Reactivity
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:5000-1:20000
  • ELISA 1:5000-1:10000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesRabbit IgG,
Cellular locationSecreted.

Documents

Certificate of Compliance

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Lot number

    ABclonal: - HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb (AS122)}

    - HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb (AS122)

    Dose response curve of HRP conjugated Mouse Anti-Rabbit IgG (Fc) mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate., blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Mouse Anti-Rabbit IgG (Fc) mAb in a 2 fold serial dilution from 2 μg/mL to 1.22*10^-4 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated thatMouse Anti-Rabbit IgG (Fc) mAb has highly specific recognition of Rabbit IgG while no or minimal cross reactivity to Rabbit IgM、 Human IgG、Mouse IgG、Rat IgG.
    ABclonal:Western blot - HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb (AS122)}

    Western blot - HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb (AS122)

    Western blot analysis of lysates from Rabbit IgG using HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb (AS122) at 1:1000-1:20000 dilution incubated overnight at 4℃.
    Lysates/proteins: 100ng per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.

    * For research use only. Not for therapeutic or diagnostic purposes.