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HRP-conjugated Mouse anti-Rabbit IgG Light Chain (AS061)

Publications (59) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:

ABclonal:HRP-conjugated Mouse anti-Rabbit IgG Light Chain
ABclonal:Western blot - HRP-conjugated Mouse anti-Rabbit IgG Light Chain (AS061)

Western blot analysis of recombinant Rabbit/Mouse/Rat control IgG Protein using HRP-conjugated Mouse Anti-Rabbit IgG Light Chain (AS061) at 1:10000 dilution.
Secondary antibody:HRP Goat Anti-Mouse IgG (H+L)(AS003) at 1:10000 dilution.
Lysates/proteins: 100 ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunoprecipitation - HRP-conjugated Mouse anti-Rabbit IgG Light Chain (AS061)

Immunoprecipitation analysis of 200 μg extracts of 293T cells using 3 μg IKKα antibody (A2062). Western blot was performed from the immunoprecipitate using IKKα antibody (A2062) at a dilution of 1:1000. Secondary antibody: HRP-conjugated AffiniPure Mouse Anti-Rabbit IgG Light Chain (AS061) at 1:20000 dilution.

Overview

Product nameHRP-conjugated Mouse anti-Rabbit IgG Light Chain
Catalog No.AS061
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG1, Kappa
CloneNo.AMC0531
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
ImmunogenRabbit IgG
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW25kDa(Light chain)
Reactivity
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:5000 - 1:10000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.75% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunoprecipitation    
Positive samplesRabbit control IgG
Cellular location
Customer validation

WB(Mus musculus, Homo sapiens, Rattus norvegicus, Sus scrofa, Danio rerio, Oryza sativa, B. mori, Mus musculus)

IHC(Homo sapiens, Mus musculus)

IP(Homo sapiens, Mus musculus)

IF(Homo sapiens)

ChIP(Other)

Co-IP(Other)

WB(Other)

Co-IP(Oryctolagus cuniculus)

IF(Zea mays)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - HRP-conjugated Mouse anti-Rabbit IgG Light Chain (AS061)}

    Western blot - HRP-conjugated Mouse anti-Rabbit IgG Light Chain (AS061)

    Western blot analysis of recombinant Rabbit/Mouse/Rat control IgG Protein using HRP-conjugated Mouse Anti-Rabbit IgG Light Chain (AS061) at 1:10000 dilution.
    Secondary antibody:HRP Goat Anti-Mouse IgG (H+L)(AS003) at 1:10000 dilution.
    Lysates/proteins: 100 ng per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunoprecipitation - HRP-conjugated Mouse anti-Rabbit IgG Light Chain (AS061)}

    Immunoprecipitation - HRP-conjugated Mouse anti-Rabbit IgG Light Chain (AS061)

    Immunoprecipitation analysis of 200 μg extracts of 293T cells using 3 μg IKKα antibody (A2062). Western blot was performed from the immunoprecipitate using IKKα antibody (A2062) at a dilution of 1:1000. Secondary antibody: HRP-conjugated AffiniPure Mouse Anti-Rabbit IgG Light Chain (AS061) at 1:20000 dilution.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AS061? Please let us know so that we can cite the reference in this datasheet.