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HRP conjugated Rabbit Anti-Monkey IgA mAb (AS116)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Cynomolgus monkey

ABclonal: - HRP conjugated Rabbit Anti-Monkey IgA mAb (AS116)

Dose response curve of HRP conjugated Monkey IgA mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate., blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Monkey IgA mAb in a 3 fold serial dilution from 1 μg/mL to 6.97×10^-2 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit anti-Monkey IgA mAb has highly specific recognition of Monkey IgA while no or minimal cross reactivity to Monkey IgD、Monkey IgE、Monkey IgG3、Human IgA、Mouse IgA.

Overview

Product nameHRP conjugated Rabbit Anti-Monkey IgA mAb
Catalog No.AS116
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgA
CloneNo.ARC59910-02-HRP
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
ImmunogenMonkey IgA
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MWRefer to figures
ReactivityCynomolgus monkey
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • ELISA 1:5000 -1:10000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application
Positive samples
Cellular location
    ABclonal: - HRP conjugated Rabbit Anti-Monkey IgA mAb (AS116)}

    - HRP conjugated Rabbit Anti-Monkey IgA mAb (AS116)

    Dose response curve of HRP conjugated Monkey IgA mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate., blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Monkey IgA mAb in a 3 fold serial dilution from 1 μg/mL to 6.97×10^-2 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit anti-Monkey IgA mAb has highly specific recognition of Monkey IgA while no or minimal cross reactivity to Monkey IgD、Monkey IgE、Monkey IgG3、Human IgA、Mouse IgA.

    * For research use only. Not for therapeutic or diagnostic purposes.