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HRP-conjugated Rabbit anti-Chicken IgY (IgG) (H+L) (AS030)

Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:

ABclonal:Western blot - HRP-conjugated Rabbit anti-Chicken IgY (IgG) (H+L) (AS030)

Western blot analysis of lysates from Chicken IgY using HRP Rabbit Anti-Chicken IgY (IgG) (H+L) (AS030) at 1:5000 dilution.
Lysates/proteins: 50ng - 100ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - HRP-conjugated Rabbit anti-Chicken IgY (IgG) (H+L) (AS030)

Western blot analysis of lysates from Chicken IgY using HRP Rabbit Anti-Chicken IgY (IgG) (H+L) (AS030) at 1:5000 dilution.
Lysates/proteins: 100ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product nameHRP-conjugated Rabbit anti-Chicken IgY (IgG) (H+L)
Catalog No.AS030
Host speciesRabbit
Purification methodAffinity purification
IsotypeHorseradish peroxidase conjugated IgG
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
ImmunogenChicken IgY (IgG)
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW22-30kDa(Light chain), 60-70kDa(Heavy chain)/30kDa(Light chain), 70kDa(Heavy chain)
Reactivity
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:10000 - 1:50000
  • IHC-P 1:500 - 1:5000
  • ELISA 1:10000 - 1:200000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.75% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesChicken IgY,
Cellular location
Customer validation

ELISA(Gallus gallus)

WB(Homo sapiens)

IP(Caenorhabditis elegans)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - HRP-conjugated Rabbit anti-Chicken IgY (IgG) (H+L) (AS030)}

    Western blot - HRP-conjugated Rabbit anti-Chicken IgY (IgG) (H+L) (AS030)

    Western blot analysis of lysates from Chicken IgY using HRP Rabbit Anti-Chicken IgY (IgG) (H+L) (AS030) at 1:5000 dilution.
    Lysates/proteins: 50ng - 100ng per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Western blot - HRP-conjugated Rabbit anti-Chicken IgY (IgG) (H+L) (AS030)}

    Western blot - HRP-conjugated Rabbit anti-Chicken IgY (IgG) (H+L) (AS030)

    Western blot analysis of lysates from Chicken IgY using HRP Rabbit Anti-Chicken IgY (IgG) (H+L) (AS030) at 1:5000 dilution.
    Lysates/proteins: 100ng per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AS030? Please let us know so that we can cite the reference in this datasheet.