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HRP-conjugated Rabbit anti HA-Tag mAb (AE123)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Western blot - HRP-conjugated Rabbit anti HA-Tag mAb (AE123)

Western blot analysis of lysates from wild type (WT) and 293F cells transfected with SCRN1-HA-GFP(N terminal) using HRP-conjugated Rabbit anti HA-Tag mAb(AE123) at 1:10000 dilution. 
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

Overview

Product nameHRP-conjugated Rabbit anti HA-Tag mAb
Catalog No.AE123
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG1, Kappa
CloneNo.ARC59578-HRP
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
ImmunogenA synthetic peptide corresponding to HA tag.
SequenceYPYDVPDYA
Gene ID
Swiss Prot
SynonymsHA; HA tag; HA-tag; HA-Tag
Calculated MW
Observed MW53kDa
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:5000 - 1:10000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293F-SCRN1-HA
Cellular location

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    ABclonal:Western blot - HRP-conjugated Rabbit anti HA-Tag mAb (AE123)}

    Western blot - HRP-conjugated Rabbit anti HA-Tag mAb (AE123)

    Western blot analysis of lysates from wild type (WT) and 293F cells transfected with SCRN1-HA-GFP(N terminal) using HRP-conjugated Rabbit anti HA-Tag mAb(AE123) at 1:10000 dilution. 
    Lysates/proteins: 30 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 5s.

    * For research use only. Not for therapeutic or diagnostic purposes.