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HRP-conjugated Rabbit anti His-Tag mAb (AE104)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Western blot - HRP-conjugated Rabbit anti His-Tag mAb (AE104)

Western blot analysis of various lysates, using HRP-conjugated Rabbit anti His-Tag mAb (AE104) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.09s.

Overview

Product nameHRP-conjugated Rabbit anti His-Tag mAb
Catalog No.AE104
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG2B, Kappa
CloneNo.ARC2696-HRP
Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected by using His antibodies.
ImmunogenA synthetic peptide corresponding to His tag.The antibody may cross-react with a protein of unknown origin ~60-70kDa.
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsHis; His tag; His-tag
Calculated MW
Observed MW17kDa(NPPA-His)/35kDa（EPCAM-His）
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:10000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.03% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T
Cellular location
Customer validation

WB(Zea mays, Mus musculus)

Documents

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    ABclonal:Western blot - HRP-conjugated Rabbit anti His-Tag mAb (AE104)}

    Western blot - HRP-conjugated Rabbit anti His-Tag mAb (AE104)

    Western blot analysis of various lysates, using HRP-conjugated Rabbit anti His-Tag mAb (AE104) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 0.09s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AE104? Please let us know so that we can cite the reference in this datasheet.