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HRP-conjugated Rabbit anti-Human IgA (Fc) mAb (AS103)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal: - HRP-conjugated Rabbit anti-Human IgA (Fc) mAb (AS103)

Dose response curve of HRP conjugated Rabbit anti-Human IgA (Fc) mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate., blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit anti-Human IgA (Fc) mAb in a 2 fold serial dilution from 1 μg/mL to 7.8*10^-3 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit Rabbit Anti-Human IgG4 has highly specific recognition of Human IgA (Fc) while no or minimal cross reactivity to Human IgG1、Human IgG2、Human IgG3、Human IgG4、Human IgM、Human IgE、Human IgD.

Overview

Product nameHRP-conjugated Rabbit anti-Human IgA (Fc) mAb
Catalog No.AS103
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC60386-HRP
Immunogen
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • ELISA 1:5000-1:10000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application
Positive samples
Cellular location
Customer validation

WB(Homo sapiens)

    ABclonal: - HRP-conjugated Rabbit anti-Human IgA (Fc) mAb (AS103)}

    - HRP-conjugated Rabbit anti-Human IgA (Fc) mAb (AS103)

    Dose response curve of HRP conjugated Rabbit anti-Human IgA (Fc) mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate., blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit anti-Human IgA (Fc) mAb in a 2 fold serial dilution from 1 μg/mL to 7.8*10^-3 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit Rabbit Anti-Human IgG4 has highly specific recognition of Human IgA (Fc) while no or minimal cross reactivity to Human IgG1、Human IgG2、Human IgG3、Human IgG4、Human IgM、Human IgE、Human IgD.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AS103? Please let us know so that we can cite the reference in this datasheet.