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HRP-conjugated Rabbit anti-Human IgG1 (Fc) mAb (AS092)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - HRP-conjugated Rabbit anti-Human IgG1 (Fc)  mAb (AS092)

Western blot analysis of various lysates, using HRP Rabbit anti-Human IgG1 (Fc) (AS092) at 1:4000-1:32000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product nameHRP-conjugated Rabbit anti-Human IgG1 (Fc) mAb
Catalog No.AS092
Host speciesRabbit
Purification methodAffinity purification
IsotypeHorseradish peroxidase conjugated IgG
CloneNo.ARC53385-HRP
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
ImmunogenHuman IgG
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW50kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:30000
  • ELISA 1:5000-1:10000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.03% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples
Cellular location

Documents

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Lot number

    ABclonal:Western blot - HRP-conjugated Rabbit anti-Human IgG1 (Fc)  mAb (AS092)}

    Western blot - HRP-conjugated Rabbit anti-Human IgG1 (Fc) mAb (AS092)

    Western blot analysis of various lysates, using HRP Rabbit anti-Human IgG1 (Fc) (AS092) at 1:4000-1:32000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.