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HRP-conjugated Rabbit anti-Human IgM mAb (AS111)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:

ABclonal: - HRP-conjugated Rabbit anti-Human IgM mAb (AS111)

Dose response curve of HRP conjugated Human IgM mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate., blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Human IgM mAb in a 3 fold serial dilution from 0.5 μg/mL to 3.05×10^-2 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit anti-Human IgM mAb has highly specific recognition of Human IgM while no or minimal cross reactivity to Human IgG1、Human IgG2、Human IgG3、Human IgG4、Mouse IgM、Rat IgM.

Overview

Product nameHRP-conjugated Rabbit anti-Human IgM mAb
Catalog No.AS111
Host speciesRabbit
Purification method
Isotype
CloneNo.ARC63173-HRP
Immunogen
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MWRefer to figures
Reactivity
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • ELISA 1:5000-1:10000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application
Positive samples
Cellular location

Documents

Certificate of Compliance

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Lot number

    ABclonal: - HRP-conjugated Rabbit anti-Human IgM mAb (AS111)}

    - HRP-conjugated Rabbit anti-Human IgM mAb (AS111)

    Dose response curve of HRP conjugated Human IgM mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate., blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Human IgM mAb in a 3 fold serial dilution from 0.5 μg/mL to 3.05×10^-2 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit anti-Human IgM mAb has highly specific recognition of Human IgM while no or minimal cross reactivity to Human IgG1、Human IgG2、Human IgG3、Human IgG4、Mouse IgM、Rat IgM.

    * For research use only. Not for therapeutic or diagnostic purposes.