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Human Activated T Cell Panel (RK40006)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Flow CytoMetry - Human Activated T Cell Panel (RK40006)

Multicolor flow cytometry panel to identify human activated T cells. Unstimulated (left panel) and stimulated (right panel) PBMCs were surface stained with ABflo® 450 Mouse anti-Human CD3 mAb (A25189, 5 μL/Test), ABflo® 610 Rabbit anti-Human CD4 mAb (A26218, 5 μL/Test), ABflo® 488 Rabbit anti-Human/Monkey CD8a mAb (A23345, 5 μL/Test), PE Rabbit anti-Human CD25 mAb (A26773, 5 μL/Test) and ABflo® 647 Rabbit anti-Human CD69 mAb (A25892, 5 μL/Test). All antibodies are validated for Flow cytometry. CD3 T lymphocytes are defined as CD3+; CD4 T cells are defined as CD4+CD3+; CD8 T cells are defined as CD8+CD3+; activated T cells are defined as CD25+CD69+.

Overview

Product nameHuman Activated T Cell Panel
Catalog No.RK40006
Host speciesRabbit
Purification method
IsotypeIgG
The 5-fluorescence Human Activated T Cell Panel has been designed for identify and phenotype CD3+ activated T cells in Human blood or tissue samples. ABflo® multi-fluorescence panel RK40006 covers the core CD markers of CD25, CD3, CD4, CD8 and CD69. Our plots showed clear subgroups of CD3+ T cells, CD4+CD3+ T cells, CD8+CD3+ T cells and CD25+CD69+ activated T cells (cite figures).
Immunogen
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular location
    ABclonal:Flow CytoMetry - Human Activated T Cell Panel (RK40006)}

    Flow CytoMetry - Human Activated T Cell Panel (RK40006)

    Multicolor flow cytometry panel to identify human activated T cells. Unstimulated (left panel) and stimulated (right panel) PBMCs were surface stained with ABflo® 450 Mouse anti-Human CD3 mAb (A25189, 5 μL/Test), ABflo® 610 Rabbit anti-Human CD4 mAb (A26218, 5 μL/Test), ABflo® 488 Rabbit anti-Human/Monkey CD8a mAb (A23345, 5 μL/Test), PE Rabbit anti-Human CD25 mAb (A26773, 5 μL/Test) and ABflo® 647 Rabbit anti-Human CD69 mAb (A25892, 5 μL/Test). All antibodies are validated for Flow cytometry. CD3 T lymphocytes are defined as CD3+; CD4 T cells are defined as CD4+CD3+; CD8 T cells are defined as CD8+CD3+; activated T cells are defined as CD25+CD69+.

    * For research use only. Not for therapeutic or diagnostic purposes.