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Human IgG4 (Fc) Rabbit mAb (A23201)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - Human IgG4 (Fc) Rabbit mAb (A23201)

Western blot analysis of various lysates, using Human IgG4 (Fc) Rabbit mAb (A23201) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - Human IgG4 (Fc) Rabbit mAb (A23201)

Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded human tonsil using Human IgG4 (Fc) Rabbit mAb (A23201) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameHuman IgG4 (Fc) Rabbit mAb
Catalog No.A23201
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC60037
As a monomeric immunoglobulin that is predominately involved in the secondary antibody response and the only isotype that can pass through the human placenta, Immunoglobulin G (IgG) is synthesized and secreted by plasma B cells, and constitutes 75% of serum immunoglobulins in humans. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as the antibody-dependent cell-mediated cytotoxicity (ADCC). IgG tetramer contains two heavy chains (50 kDa ) and two light chains (25 kDa) linked by disulfide bonds, that is the two identical halves form the Y-like shape. IgG is digested by pepsin proteolysis into Fab fragment (antigen-binding fragment) and Fc fragment ("crystallizable" fragment).
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 96-115 of Human IgG4 (Fc). (P01861).
SequenceKRVESKYGPPCPSCPAPEFL
Gene ID
Swiss Prot
SynonymsIGHG4; Ig gamma-4 chain C region; Human IgG4 (Fc)
Calculated MW36kDa
Observed MW55kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IF/ICC 1:100 - 1:500
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHuman plasma, HeLa(Negative control)
Cellular locationSecreted

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Human IgG4 (Fc) Rabbit mAb (A23201)}

    Western blot - Human IgG4 (Fc) Rabbit mAb (A23201)

    Western blot analysis of various lysates, using Human IgG4 (Fc) Rabbit mAb (A23201) at 1:2000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunofluorescence - Human IgG4 (Fc) Rabbit mAb (A23201)}

    Immunofluorescence - Human IgG4 (Fc) Rabbit mAb (A23201)

    Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded human tonsil using Human IgG4 (Fc) Rabbit mAb (A23201) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.