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Human IgG4/Immunoglobulin heavy constant gamma 4 Rabbit pAb (A24655)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - Human IgG4/Immunoglobulin heavy constant gamma 4 Rabbit pAb (A24655)

Western blot analysis of various lysates, using Human IgG4/Immunoglobulin heavy constant gamma 4 Rabbit pAb (A24655) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product nameHuman IgG4/Immunoglobulin heavy constant gamma 4 Rabbit pAb
Catalog No.A24655
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Immunoglobulin G (IgG), is one of the most abundant protein in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B-lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 50-150 of human IgG4/Immunoglobulin heavy constant gamma 4 (P01861).
SequenceVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKRVESKYGPPCPSCPAPEFLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSQED
Gene ID
Swiss Prot
SynonymsIGHG4; Ig gamma-4 chain C region; Human IgG4/Immunoglobulin heavy constant gamma 4
Calculated MW44kDa
Observed MW36kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesRPMI-8226, SH-SY5Y, Daudi, U-251 MG, K-562
Cellular locationSecreted

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    ABclonal:Western blot - Human IgG4/Immunoglobulin heavy constant gamma 4 Rabbit pAb (A24655)}

    Western blot - Human IgG4/Immunoglobulin heavy constant gamma 4 Rabbit pAb (A24655)

    Western blot analysis of various lysates, using Human IgG4/Immunoglobulin heavy constant gamma 4 Rabbit pAb (A24655) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.