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Human Lambda Light Chain Rabbit mAb (A24615)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - Human Lambda Light Chain Rabbit mAb (A24615)

Western blot analysis of various lysates, using Human Lambda Light Chain Rabbit mAb (A24615) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): HAP1
Exposure time: 0.2s.

ABclonal:Immunohistochemistry - Human Lambda Light Chain Rabbit mAb (A24615)

Immunohistochemistry analysis of paraffin-embedded Human tonsil using Human Lambda Light Chain Rabbit mAb (A24615) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Human Lambda Light Chain Rabbit mAb (A24615)

Immunofluorescence analysis of paraffin-embedded human tonsil using Human Lambda Light Chain Rabbit mAb (A24615) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Overview

Product nameHuman Lambda Light Chain Rabbit mAb
Catalog No.A24615
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC60732
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-106 of human Lambda Light Chain.
SequenceGQPKANPTVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADGSPVKAGVETTKPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS
Gene ID
Swiss Prot
Synonyms
Calculated MW11kDa
Observed MW30kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:10000
  • IHC-P 1:200 - 1:2000
  • IF/ICC 1:100 - 1:800
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHuman plasma
Cellular location

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Human Lambda Light Chain Rabbit mAb (A24615)}

    Western blot - Human Lambda Light Chain Rabbit mAb (A24615)

    Western blot analysis of various lysates, using Human Lambda Light Chain Rabbit mAb (A24615) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Negative control (NC): HAP1
    Exposure time: 0.2s.
    ABclonal:Immunohistochemistry - Human Lambda Light Chain Rabbit mAb (A24615)}

    Immunohistochemistry - Human Lambda Light Chain Rabbit mAb (A24615)

    Immunohistochemistry analysis of paraffin-embedded Human tonsil using Human Lambda Light Chain Rabbit mAb (A24615) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunofluorescence - Human Lambda Light Chain Rabbit mAb (A24615)}

    Immunofluorescence - Human Lambda Light Chain Rabbit mAb (A24615)

    Immunofluorescence analysis of paraffin-embedded human tonsil using Human Lambda Light Chain Rabbit mAb (A24615) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

    * For research use only. Not for therapeutic or diagnostic purposes.