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Human Treg Panel (RK40005)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Flow CytoMetry - Human Treg Panel (RK40005)

Multicolor flow cytometry panel to identify human regulatory T cells.PBMCs were surface stained with ABflo® 594 Rabbit anti-Human CD4 mAb (A25282, 5 μL/Test), APC Rabbit anti-Human CD25 mAb (A26774, 5 μL/Test) and ABflo® 488 Rabbit anti-Human CD127/IL-7Rα mAb (A25161, 5 μL/Test). All antibodies are validated for Flow cytometry. CD4 T cells are defined as CD4+; regulatory T cells are defined as CD25+CD127low/-.

Overview

Product nameHuman Treg Panel
Catalog No.RK40005
Host speciesRabbit
Purification method
IsotypeIgG
The 3-fluorescence Human Treg Panel has been designed for identify and phenotype CD4+ T regulatory cells in Human blood or tissue samples. ABflo® multi-fluorescence panel RK40005 covers the core CD markers of CD4, CD25 and CD127. Our plots showed clear subgroups of CD4+ T cells, and CD25+CD127low/- Treg cells (cite figures).
Immunogen
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular location
    ABclonal:Flow CytoMetry - Human Treg Panel (RK40005)}

    Flow CytoMetry - Human Treg Panel (RK40005)

    Multicolor flow cytometry panel to identify human regulatory T cells.PBMCs were surface stained with ABflo® 594 Rabbit anti-Human CD4 mAb (A25282, 5 μL/Test), APC Rabbit anti-Human CD25 mAb (A26774, 5 μL/Test) and ABflo® 488 Rabbit anti-Human CD127/IL-7Rα mAb (A25161, 5 μL/Test). All antibodies are validated for Flow cytometry. CD4 T cells are defined as CD4+; regulatory T cells are defined as CD25+CD127low/-.

    * For research use only. Not for therapeutic or diagnostic purposes.