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K29-linkage Specific Polyubiquitin Rabbit pAb (A18198)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

ABclonal:Western blot - K29-linkage Specific Polyubiquitin Rabbit pAb (A18198)

Western blot analysis of various lysates using K29-linkage Specific Polyubiquitin Rabbit pAb (A18198) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Dot Blot - K29-linkage Specific Polyubiquitin Rabbit pAb (A18198)

Dot-blot analysis of all sorts of peptides using K29-linkage Specific Polyubiquitin antibody (A18198) at 1:1000 dilution.

Overview

Product nameK29-linkage Specific Polyubiquitin Rabbit pAb
Catalog No.A18198
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Polyubiquitin chains regulate diverse cellular processes through the ability of ubiquitin to form chains of eight different linkage types. when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked. K29-linked ubiquitination is involved in different kinds of cellular proteotoxic stress response as well as cell cycle regulation. In particular, K29-linked ubiquitination is enriched in the midbody and downregulation of the K29-linked ubiquitination signal arrests cells in G1/S phase.
ImmunogenA synthetic peptide corresponding to a sequence containing polyubiquitinated protein (K29 linkage).
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW
ReactivityHuman, Mouse, Rat, Other (Wide Range Predicted)
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • DB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, NIH/3T3, RAW264.7, C6, Mouse brain, Mouse kidney, Rat brain, Rat kidney
Cellular location
Customer validation

Other(Oryza sativa)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - K29-linkage Specific Polyubiquitin Rabbit pAb (A18198)}

    Western blot - K29-linkage Specific Polyubiquitin Rabbit pAb (A18198)

    Western blot analysis of various lysates using K29-linkage Specific Polyubiquitin Rabbit pAb (A18198) at 1:500 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 90s.
    ABclonal:Dot Blot - K29-linkage Specific Polyubiquitin Rabbit pAb (A18198)}

    Dot Blot - K29-linkage Specific Polyubiquitin Rabbit pAb (A18198)

    Dot-blot analysis of all sorts of peptides using K29-linkage Specific Polyubiquitin antibody (A18198) at 1:1000 dilution.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A18198? Please let us know so that we can cite the reference in this datasheet.