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K33-linkage Specific Polyubiquitin Rabbit pAb (A18199)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - K33-linkage Specific Polyubiquitin Rabbit pAb (A18199)

Western blot analysis of various lysates using K33-linkage Specific Polyubiquitin Rabbit pAb (A18199) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Dot Blot - K33-linkage Specific Polyubiquitin Rabbit pAb (A18199)

Dot-blot analysis of all sorts of peptides using K33-linkage Specific Polyubiquitin antibody (A18199) at 1:1000 dilution.

Overview

Product nameK33-linkage Specific Polyubiquitin Rabbit pAb
Catalog No.A18199
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. An aberrant form of this protein has been detected in patients with Alzheimer's disease and Down syndrome. Pseudogenes of this gene are located on chromosomes 1, 2, 13, and 17. Alternative splicing results in multiple transcript variants.
ImmunogenA synthetic peptide corresponding to a sequence containing polyubiquitinated protein (K33 linkage).
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • DB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, NIH/3T3, RAW264.7, C6
Cellular location
Customer validation

Other(Oryza sativa)

WB(Mus musculus)

Documents

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Lot number

    ABclonal:Western blot - K33-linkage Specific Polyubiquitin Rabbit pAb (A18199)}

    Western blot - K33-linkage Specific Polyubiquitin Rabbit pAb (A18199)

    Western blot analysis of various lysates using K33-linkage Specific Polyubiquitin Rabbit pAb (A18199) at 1:500 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 90s.
    ABclonal:Dot Blot - K33-linkage Specific Polyubiquitin Rabbit pAb (A18199)}

    Dot Blot - K33-linkage Specific Polyubiquitin Rabbit pAb (A18199)

    Dot-blot analysis of all sorts of peptides using K33-linkage Specific Polyubiquitin antibody (A18199) at 1:1000 dilution.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A18199? Please let us know so that we can cite the reference in this datasheet.