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K63-linkage Specific Polyubiquitin Rabbit pAb (A18164)

Publications (7) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

ABclonal:Dot Blot - K63-linkage Specific Polyubiquitin Rabbit pAb (A18164)

Dot-blot analysis of all sorts of peptides using K63-linkage Specific Polyubiquitin antibody (A18164) at 1:1000 dilution.

Overview

Product nameK63-linkage Specific Polyubiquitin Rabbit pAb
Catalog No.A18164
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Ubiquitination, onetypeofthemostcommonpost-translationalmodification, mediatestheregulationofproteinhomeostasisinvivo.SinceubiquitinitselfcontainsmultiplelysineresiduesandoneN-terminalfreeaminogroup, eighttypesofubiquitinchainscanbeformed.? K63-linked polyubiquitin chains exert nonproteolytic functions in vivo, such as protein trafficking, kinase/phosphatase activation, and DNA damage control, all of which might be important in regulation of cancer survival and development （PMID: 19217402，PMCID: PMC3008764）.
ImmunogenA synthetic peptide corresponding to a sequence containing polyubiquitinated protein (K63 linkage).
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW15-200kDa
ReactivityHuman, Mouse, Rat, Other (Wide Range Predicted)
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • DB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application
Positive samplesHeLa, HeLa+MG132, NIH/3T3, C6, Mouse brain, Rat brain
Cellular location
Customer validation

WB(Drosophila melanogaster, Mus musculus, Homo sapiens)

Other(Oryza sativa)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Dot Blot - K63-linkage Specific Polyubiquitin Rabbit pAb (A18164)}

    Dot Blot - K63-linkage Specific Polyubiquitin Rabbit pAb (A18164)

    Dot-blot analysis of all sorts of peptides using K63-linkage Specific Polyubiquitin antibody (A18164) at 1:1000 dilution.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A18164? Please let us know so that we can cite the reference in this datasheet.