제품 > 항체 > Other Modified Antibodies

K6-linkage Specific Polyubiquitin Rabbit pAb (A18106)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - K6-linkage Specific Polyubiquitin Rabbit pAb (A18106)

Western blot analysis of various lysates using K6-linkage Specific Polyubiquitin Rabbit pAb (A18106) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Dot Blot - K6-linkage Specific Polyubiquitin Rabbit pAb (A18106)

Dot-blot analysis of all sorts of peptides using K6-linkage Specific Polyubiquitin antibody (A18106) at 1:1000 dilution.

Overview

Product nameK6-linkage Specific Polyubiquitin Rabbit pAb
Catalog No.A18106
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Polyubiquitin chains regulate diverse cellular processes through the ability of ubiquitin to form chains of eight different linkage types. Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair. it has been reported that modification at Lys-6 inhibits ubiquitin-dependent protein degradation, a failure of the ubiquitin-proteasome system could play a role in initiating the formation of degradation-resistant PHF tangles.
ImmunogenA synthetic peptide corresponding to a sequence containing polyubiquitinated protein (K6 linkage).
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • DB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesRAW264.7, C6
Cellular location
Customer validation

Other(Oryza sativa)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - K6-linkage Specific Polyubiquitin Rabbit pAb (A18106)}

    Western blot - K6-linkage Specific Polyubiquitin Rabbit pAb (A18106)

    Western blot analysis of various lysates using K6-linkage Specific Polyubiquitin Rabbit pAb (A18106) at 1:500 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 180s.
    ABclonal:Dot Blot - K6-linkage Specific Polyubiquitin Rabbit pAb (A18106)}

    Dot Blot - K6-linkage Specific Polyubiquitin Rabbit pAb (A18106)

    Dot-blot analysis of all sorts of peptides using K6-linkage Specific Polyubiquitin antibody (A18106) at 1:1000 dilution.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A18106? Please let us know so that we can cite the reference in this datasheet.