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Magnetic Beads-conjugated Mouse anti DDDDK-Tag mAb (AE037)

Publications (6) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Magnetic Beads-conjugated Mouse anti DDDDK-Tag mAb
ABclonal:Immunoprecipitation - Magnetic Beads-conjugated Mouse anti DDDDK-Tag mAb (AE037)

Immunoprecipitation analysis of 300 ug extract cell lysate from 293T cells transfected with GSK3B expression vector containing a C-terminal DDDDK-Tag (1x) with 30uL Magnetic Beads-conjugated Mouse anti DDDDK-tag mAb antibody（AE037). Magnetic Beads-conjugated mouse IgG isotype control pAb (AC044N) was used as a negative control. Western blot was performed from the immunoprecipitate using Rabbit anti DDDDK-Tag mAb antibody (AE092) at 1:10000 dilution.

ABclonal:Immunoprecipitation - Magnetic Beads-conjugated Mouse anti DDDDK-Tag mAb (AE037)

Immunoprecipitation analysis of 600ug extract cell lysate from 293T transfected with SERPINB1 expression vector containing a N-terminal DDDDK-Tag (1x) with 30ul Magnetic Beads-conjugated Mouse anti DDDDK-Tag antibody (AE037). Magnetic Beads-conjugated mouse IgG isotype control pAb (AC044N) was used as a negative control. Western blot was performed from the immunoprecipitate using Rabbit anti DDDDK-Tag mAb antibody (AE092) at 1:5000 dilution.

Overview

Product nameMagnetic Beads-conjugated Mouse anti DDDDK-Tag mAb
Catalog No.AE037
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG1
CloneNo.AMC0515
FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography.A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis and Western blotting.
ImmunogenA synthetic peptide corresponding to DDDDK tag.
SequenceDYKDDDDK
Gene ID
Swiss Prot
SynonymsDDDDK; DDDDK tag; DDDDK-tag
Calculated MW
Observed MW50kDa/48kDa
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IP 30ul antibody (bead slurry) for 200μg-400μg extracts of whole cells
Storage bufferStore at 4℃ Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, pH7.3.
Key applicationImmunoprecipitation    
Positive samples293T-SERPINB1-Flag (N-terminal) , 293T-GSK3B-Flag(C-terminal)
Cellular location
Customer validation

Co-IP(Other, Cherax quadricarinatus, Nicotiana benthamiana)

IP(Cyprinus carpio, Homo sapiens, Escherichia coli)

Documents

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    ABclonal:Immunoprecipitation - Magnetic Beads-conjugated Mouse anti DDDDK-Tag mAb (AE037)}

    Immunoprecipitation - Magnetic Beads-conjugated Mouse anti DDDDK-Tag mAb (AE037)

    Immunoprecipitation analysis of 300 ug extract cell lysate from 293T cells transfected with GSK3B expression vector containing a C-terminal DDDDK-Tag (1x) with 30uL Magnetic Beads-conjugated Mouse anti DDDDK-tag mAb antibody（AE037). Magnetic Beads-conjugated mouse IgG isotype control pAb (AC044N) was used as a negative control. Western blot was performed from the immunoprecipitate using Rabbit anti DDDDK-Tag mAb antibody (AE092) at 1:10000 dilution.
    ABclonal:Immunoprecipitation - Magnetic Beads-conjugated Mouse anti DDDDK-Tag mAb (AE037)}

    Immunoprecipitation - Magnetic Beads-conjugated Mouse anti DDDDK-Tag mAb (AE037)

    Immunoprecipitation analysis of 600ug extract cell lysate from 293T transfected with SERPINB1 expression vector containing a N-terminal DDDDK-Tag (1x) with 30ul Magnetic Beads-conjugated Mouse anti DDDDK-Tag antibody (AE037). Magnetic Beads-conjugated mouse IgG isotype control pAb (AC044N) was used as a negative control. Western blot was performed from the immunoprecipitate using Rabbit anti DDDDK-Tag mAb antibody (AE092) at 1:5000 dilution.

    * For research use only. Not for therapeutic or diagnostic purposes.

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