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Magnetic Beads-conjugated Rabbit IgG isotype control (AC047)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:

ABclonal:Immunoprecipitation - Magnetic Beads-conjugated Rabbit IgG isotype control (AC047)

Immunoprecipitation analysis of 300 ug extract cell lysate from 293T cells transfected with GSK3B expression vector containing a C-terminal DDDDK tag (1x) with 30uL Magnetic Beads-conjugated Rabbit anti-DDDDK-tag mAb antibody（AE097). Magnetic Beads-conjugated Rabbit IgG isotype control mAb was used as a negative control（AC047 ). Western blot was performed from the immunoprecipitate using Mouse anti DDDDK-Tag mAb antibody (AE005) at 1:2000 dilution.Secondary antibody: HRP-conjugated AffiniPure Goat Anti-Mouse IgG Light Chain (AS062).

Overview

Product nameMagnetic Beads-conjugated Rabbit IgG isotype control
Catalog No.AC047
Host speciesRabbit
Purification methodProtein A/G purification
IsotypeIgG
CloneNo.ARC5105-02-Beads
ImmunogenNone
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW
Reactivity
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IP 30ul antibody (bead slurry) for 200μg-400μg extracts of whole cells
Storage bufferStore at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, pH7.3.
Key applicationImmunoprecipitation    
Positive samples
Cellular location
Customer validation

WB(Rattus norvegicus)

    ABclonal:Immunoprecipitation - Magnetic Beads-conjugated Rabbit IgG isotype control (AC047)}

    Immunoprecipitation - Magnetic Beads-conjugated Rabbit IgG isotype control (AC047)

    Immunoprecipitation analysis of 300 ug extract cell lysate from 293T cells transfected with GSK3B expression vector containing a C-terminal DDDDK tag (1x) with 30uL Magnetic Beads-conjugated Rabbit anti-DDDDK-tag mAb antibody（AE097). Magnetic Beads-conjugated Rabbit IgG isotype control mAb was used as a negative control（AC047 ). Western blot was performed from the immunoprecipitate using Mouse anti DDDDK-Tag mAb antibody (AE005) at 1:2000 dilution.Secondary antibody: HRP-conjugated AffiniPure Goat Anti-Mouse IgG Light Chain (AS062).

    * For research use only. Not for therapeutic or diagnostic purposes.

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