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Magnetic Beads-conjugated Rabbit anti GST-Tag mAb (AE122)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Immunoprecipitation - Magnetic Beads-conjugated Rabbit anti GST-Tag mAb (AE122)

Immunoprecipitation of GST-Tag from 300 µg extracts of 293F cells transfected with a MYOD1 expression vector containing a single N-terminal GST-Tag was performed using 20 µl of Magnetic Beads-conjugated Rabbit anti GST-Tag mAb (AE122). Magnetic Beads-conjugated Rabbit IgG isotype control(AC047) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10 % of the total input. Western blot analysis of immunoprecipitates was conducted using Rabbit anti GST-Tag mAb (AE077) at a dilution of 1:2000.

Overview

Product nameMagnetic Beads-conjugated Rabbit anti GST-Tag mAb
Catalog No.AE122
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Glutathione S-transferases (GSTs), previously known as ligandins, comprise a family of eukaryotic and prokaryotic phase II metabolic isozymes best known for their ability to catalyze the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates for the purpose of detoxification. The GST family consists of three superfamilies: the cytosolic, mitochondrial, and microsomal—also known as MAPEG—proteins. Members of the GST superfamily are extremely diverse in amino acid sequence, and a large fraction of the sequences deposited in public databases are of unknown function. The Enzyme Function Initiative (EFI) is using GSTs as a model superfamily to identify new GST functions.A GST-tag is often used to separate and purify proteins that contain the GST-fusion protein. The tag is 220 amino acids (roughly 26 KDa) in size, which, compared to tags such as the Myc-tag or the FLAG-tag, is quite large. It can be fused to either the N-terminus or C-terminus of a protein. However, many commercially available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-218 of schja GST protein (P08515).
SequenceSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK
Gene ID
Swiss Prot
SynonymsGST; GST tag; GST-Tag
Calculated MW
Observed MW70kDa
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IP 20μl-40μl Magnetic Beads for 100μg-300μg extracts of whole cells
Storage bufferStore at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, pH7.3.
Key applicationImmunoprecipitation    
Positive samples293F-MYOD1-GST-Tag
Cellular location

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    ABclonal:Immunoprecipitation - Magnetic Beads-conjugated Rabbit anti GST-Tag mAb (AE122)}

    Immunoprecipitation - Magnetic Beads-conjugated Rabbit anti GST-Tag mAb (AE122)

    Immunoprecipitation of GST-Tag from 300 µg extracts of 293F cells transfected with a MYOD1 expression vector containing a single N-terminal GST-Tag was performed using 20 µl of Magnetic Beads-conjugated Rabbit anti GST-Tag mAb (AE122). Magnetic Beads-conjugated Rabbit IgG isotype control(AC047) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10 % of the total input. Western blot analysis of immunoprecipitates was conducted using Rabbit anti GST-Tag mAb (AE077) at a dilution of 1:2000.

    * For research use only. Not for therapeutic or diagnostic purposes.