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Magnetic Beads-conjugated Rabbit anti HA-Tag mAb (AE124)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Immunoprecipitation - Magnetic Beads-conjugated Rabbit anti HA-Tag mAb (AE124)

Immunoprecipitation of LDHA-3X HA in 300 µg extracts from 293T cells transfected with LDHA-3X HA using 20 µl Magnetic Beads-conjugated Rabbit anti HA-Tag mAb (AE124). Western blot analysis was performed using HRP-conjugated Mouse anti HA-Tag mAb (AE025) at 1:5000 dilution.

Overview

Product nameMagnetic Beads-conjugated Rabbit anti HA-Tag mAb
Catalog No.AE124
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG1, Kappa
CloneNo.ARC59578-MB
ImmunogenA synthetic peptide corresponding to HA tag.
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW37kDa
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IP 20μl-40μl Magnetic Beads for 100μg-300μg extracts of whole cells
Storage bufferStore at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, pH7.3.
Key applicationImmunoprecipitation    
Positive samples293T-LDHA-3xHA(N端)
Cellular location
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Immunoprecipitation - Magnetic Beads-conjugated Rabbit anti HA-Tag mAb (AE124)}

    Immunoprecipitation - Magnetic Beads-conjugated Rabbit anti HA-Tag mAb (AE124)

    Immunoprecipitation of LDHA-3X HA in 300 µg extracts from 293T cells transfected with LDHA-3X HA using 20 µl Magnetic Beads-conjugated Rabbit anti HA-Tag mAb (AE124). Western blot analysis was performed using HRP-conjugated Mouse anti HA-Tag mAb (AE025) at 1:5000 dilution.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AE124? Please let us know so that we can cite the reference in this datasheet.