제품 > 항체 > Tag & 로딩 컨트롤 항체

Magnetic beads-conjugated anti-GFP VHH Single Domain antibody (AE079)

Publications (14) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Immunoprecipitation - Magnetic beads-conjugated anti-GFP VHH Single Domain antibody (AE079)

Immunoprecipitation of SERPINB1-GFP from 200 µg extracts of 293T cells transfected with a SERPINB1 expression vector containing a single C-terminal GFP-Tag was performed using 40 µl of Magnetic beads-conjugated anti-GFP VHH Single Domain antibody (AE079). Magnetic Beads-conjugated mouse IgG isotype control (AC044N) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10 % of the total input. Western blot analysis of immunoprecipitates was conducted using Mouse anti DDDDK-Tag mAb (AE005) at a dilution of 1 : 5000.

Overview

Product nameMagnetic beads-conjugated anti-GFP VHH Single Domain antibody
Catalog No.AE079
Host speciesAlpaca
Purification methodAffinity purification
IsotypeVHH
The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield (QY) of GFP is 0.79. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 499 nm. GFP makes for an excellent tool in many forms of biology due to its ability to form internal chromophore without requiring any accessory cofactors, gene products, or enzymes / substrates other than molecular oxygen.In cell and molecular biology, the GFP gene is frequently used as a reporter of expression.
ImmunogenRecombinant protein of GFP.
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsGFP; GFP tag; GFP-tag
Calculated MW27kDa
Observed MW30kDa/68KD
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • ChIP 500 µL (20 reactions)
  • Binding Cap 0.4 mg GFP protein/mL
  • CoIP 500 µL (20 reactions)
  • RIP 500 µL (20 reactions)
Storage bufferStore at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.03% NaN3, 20% ethanol.
Key applicationImmunoprecipitation    
Positive samples
Cellular location
Customer validation

Co-IP(Arabidopsis thaliana, Homo sapiens, Oryza sativa, Crassostrea gigas)

WB(Homo sapiens, Arabidopsis thaliana, Oryza sativa)

Pull-down(Homo sapiens)

IP(Arabidopsis thaliana)

IP(Homo sapiens, Oryza sativa)

Co-IP(Oryza sativa)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Immunoprecipitation - Magnetic beads-conjugated anti-GFP VHH Single Domain antibody (AE079)}

    Immunoprecipitation - Magnetic beads-conjugated anti-GFP VHH Single Domain antibody (AE079)

    Immunoprecipitation of SERPINB1-GFP from 200 µg extracts of 293T cells transfected with a SERPINB1 expression vector containing a single C-terminal GFP-Tag was performed using 40 µl of Magnetic beads-conjugated anti-GFP VHH Single Domain antibody (AE079). Magnetic Beads-conjugated mouse IgG isotype control (AC044N) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10 % of the total input. Western blot analysis of immunoprecipitates was conducted using Mouse anti DDDDK-Tag mAb (AE005) at a dilution of 1 : 5000.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AE079? Please let us know so that we can cite the reference in this datasheet.