제품 > 항체 > Tag & 로딩 컨트롤 항체

Mouse Control IgG (AC011)

Publications (79) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:

ABclonal:Mouse Control IgG
ABclonal:Immunoprecipitation - Mouse Control IgG (AC011)

Immunoprecipitation of over-expressed Myc-tagged protein in 293T cells incubated using Myc-tag antibody (AE010). A mock served as negative control using mouse Control IgG（AC011）and over-expressed 293T cell lysate served as positive control.

Overview

Product nameMouse Control IgG
Catalog No.AC011
Host speciesMouse
Purification methodPotein A/G purification
IsotypeIgG
Mouse IgG raised from normal mouse serum. Mouse Control IgG is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. Mouse IgG typically constitutes 75% of serum immunoglobulins. Mouse IgG molecules are synthesized and secreted by plasma B cells. Ideal as a negative control for immunoprecipitation applications.
ImmunogenNone
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW
Reactivity
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IP 0.5ug-4ug antibody for 200ug-400ug extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationImmunoprecipitation    
Positive samples293T
Cellular location
Customer validation

WB(Homo sapiens, Bos taurus, Mus musculus, Helicoverpa armigera, Mus musculus)

IP(Homo sapiens, Mus musculus, Anatinae)

Co-IP(Homo sapiens, Mus musculus, Rattus norvegicus, Sus scrofa, Other)

IF(Mus musculus, Rattus norvegicus)

Injection(Mus musculus)

ChIP(Homo sapiens, Other, Drosophila melanogaster)

IF(Homo sapiens)

Co-IP(Homo sapiens)

RIP(Drosophila melanogaster, Homo sapiens, Mus musculus)

RIP(Mus musculus)

IHC(Mus musculus)

MeRIP(Mus musculus)

FC(Homo sapiens)

Other(Homo sapiens)

RIP(Mus musculus)

WB(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Immunoprecipitation - Mouse Control IgG (AC011)}

    Immunoprecipitation - Mouse Control IgG (AC011)

    Immunoprecipitation of over-expressed Myc-tagged protein in 293T cells incubated using Myc-tag antibody (AE010). A mock served as negative control using mouse Control IgG（AC011）and over-expressed 293T cell lysate served as positive control.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AC011? Please let us know so that we can cite the reference in this datasheet.