제품 > 항체 > 단일클론항체(mAb)

Mouse IgE Rabbit mAb (A23195)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Western blot - Mouse IgE Rabbit mAb (A23195)

Western blot analysis of various lysates using Mouse IgE Rabbit mAb (A23195) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Negative control (NC): Mouse brain
Exposure time: 120s.

Overview

Product nameMouse IgE Rabbit mAb
Catalog No.A23195
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC60081
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 50-388 of mouse IgE. (AAZ05128.1).
SequenceFNESRTILVRPVNITEPTLELLHSSCDPNAFHSTIQLYCFIYGHILNDVSVSWLMDDREITDTLAQTVLIKEEGKLASTCSKLNITEQQWMSESTFTCKVTSQGVDYLAHTRRCPDHEPRGVITYLIPPSPLDLYQNGAPKLTCLVVDLESEKNVNVTWNQEKKTSVSASQWYTKHHNNATTSITSILPVVAKDWIEGYGYQCIVDHPDFPKPIVRSITKTPGQRSAPEVYVFPPPEEESEDKRTLTCLIQNFFPEDISVQWLGDGKLISNSQHSTTTPLKSNGSNQGFFIFSRLEVAKTLWTQRKQFTCQVIHEALQKPRKLEKTISTSLGNTSLRPS
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW75kDa
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse lung
Cellular location

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Mouse IgE Rabbit mAb (A23195)}

    Western blot - Mouse IgE Rabbit mAb (A23195)

    Western blot analysis of various lysates using Mouse IgE Rabbit mAb (A23195) at 1:2000 dilution.
    Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Negative control (NC): Mouse brain
    Exposure time: 120s.

    * For research use only. Not for therapeutic or diagnostic purposes.