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Mouse Treg Panel (RK40004)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Flow CytoMetry - Mouse Treg Panel (RK40004)

Multicolor flow cytometry panel to identify mouse regulatory T cells. C57 BL/6 mouse splenocytes were surface-stained with ABflo® 594 Rabbit anti-Mouse CD4 mAb (A24722, 5 μL/Test) and ABflo® 647 Rabbit anti-Mouse CD25 mAb (A23808, 5 μL/Test), and then intracellularly-stained with PE Rabbit anti-Mouse FOXP3 mAb (A26227, 5 μL/Test) . All antibodies are validated for Flow cytometry. CD4 T cells are defined as CD4+; Regulatory T cells are defined as CD25+ FOXP3+.

Overview

Product nameMouse Treg Panel
Catalog No.RK40004
Host speciesRabbit
Purification method
IsotypeIgG
The 3-fluorescence Mouse Treg Panel has been designed for identify and phenotype CD4+ T regulatory cells in Mouse blood or tissue samples. ABflo® multi-fluorescence panel RK40004 covers the core CD markers of FOXP3, CD4 and CD25. Our plots showed clear subgroups of CD4+ T cells, and CD25+ FOXP3+ Treg cells (cite figures).
Immunogen
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular location
    ABclonal:Flow CytoMetry - Mouse Treg Panel (RK40004)}

    Flow CytoMetry - Mouse Treg Panel (RK40004)

    Multicolor flow cytometry panel to identify mouse regulatory T cells. C57 BL/6 mouse splenocytes were surface-stained with ABflo® 594 Rabbit anti-Mouse CD4 mAb (A24722, 5 μL/Test) and ABflo® 647 Rabbit anti-Mouse CD25 mAb (A23808, 5 μL/Test), and then intracellularly-stained with PE Rabbit anti-Mouse FOXP3 mAb (A26227, 5 μL/Test) . All antibodies are validated for Flow cytometry. CD4 T cells are defined as CD4+; Regulatory T cells are defined as CD25+ FOXP3+.

    * For research use only. Not for therapeutic or diagnostic purposes.