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Mouse anti GST-Tag mAb (AE001)

Publications (94) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Mouse anti GST-Tag mAb
ABclonal:Western blot - Mouse anti GST-Tag mAb (AE001)

Western blot analysis of over-expressed GST protein using Mouse anti GST-Tag mAb (AE001) at different dilution. Each lane was loaded with 2 ug cell lysate.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunoprecipitation - Mouse anti GST-Tag mAb (AE001)

Immunoprecipitation analysis of 1 μg extracts of GST-Tag cells using 3 μg Mouse anti GST-Tag mAb antibody (AE001). Western blot was performed from the immunoprecipitate using Mouse anti GST-Tag mAb antibody (AE001) at a dilution of 1:1000.

Overview

Product nameMouse anti GST-Tag mAb
Catalog No.AE001
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG1
CloneNo.AMC0501
Glutathione S-transferases (GSTs), previously known as ligandins, comprise a family of eukaryotic and prokaryotic phase II metabolic isozymes best known for their ability to catalyze the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates for the purpose of detoxification. The GST family consists of three superfamilies: the cytosolic, mitochondrial, and microsomal—also known as MAPEG—proteins. Members of the GST superfamily are extremely diverse in amino acid sequence, and a large fraction of the sequences deposited in public databases are of unknown function. The Enzyme Function Initiative (EFI) is using GSTs as a model superfamily to identify new GST functions.A GST-tag is often used to separate and purify proteins that contain the GST-fusion protein. The tag is 220 amino acids (roughly 26 KDa) in size, which, compared to tags such as the Myc-tag or the FLAG-tag, is quite large. It can be fused to either the N-terminus or C-terminus of a protein. However, many commercially available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification.
ImmunogenRecombinant protein containing a sequence corresponding to amino acids 1-218 of GST protein.
SequenceMSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK
Gene ID
Swiss Prot
SynonymsGST; GST tag; GST-tag
Calculated MW26kDa
Observed MW27kDa
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:5000
  • IP 3μg antibody for 1μg extracts of recombinant protein
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 1.5% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunoprecipitation    
Positive samplesGST-Tag
Cellular location
Customer validation

WB(Homo sapiens, Oryza sativa, Gallus gallus, Yeast, Other, Arabidopsis thaliana, Botrytis cinerea, Escherichia coli, Malus pumila, Malus domestica, Zea mays, Sus scrofa, Plutella xylostella, Glycine max, Mus musculus, Caenorhabditis elegans, Mus musculus, Lycopersicon esculentum, Ctenopharyngodon idella, Chlorocebus aethiops)

ELISA(Mus musculus,Rattus norvegicus,Oyster, Homo sapiens)

IF(Mus musculus,Rattus norvegicus,Oyster)

IP(Mus musculus, Oryza sativa, Helicoverpa armigera, Other, Arabidopsis thaliana, Glycine max, Homo sapiens)

IP(Arabidopsis thaliana, Other, Mus musculus, Escherichia coli, Homo sapiens, Chlorocebus aethiops)

Co-IP(Other, Ctenopharyngodon idellus)

WB(Glycine max)

Other(Other, Oryza sativa, Arabidopsis thaliana, Homo sapiens)

ChIP(Glycine max)

IF(Mus musculus, Homo sapiens)

Pull-down(Oryza sativa, Gossypium spp, Arabidopsis thaliana)

IF(Homo sapiens)

Documents

Certificate of Compliance

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    ABclonal:Western blot - Mouse anti GST-Tag mAb (AE001)}

    Western blot - Mouse anti GST-Tag mAb (AE001)

    Western blot analysis of over-expressed GST protein using Mouse anti GST-Tag mAb (AE001) at different dilution. Each lane was loaded with 2 ug cell lysate.
    Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunoprecipitation - Mouse anti GST-Tag mAb (AE001)}

    Immunoprecipitation - Mouse anti GST-Tag mAb (AE001)

    Immunoprecipitation analysis of 1 μg extracts of GST-Tag cells using 3 μg Mouse anti GST-Tag mAb antibody (AE001). Western blot was performed from the immunoprecipitate using Mouse anti GST-Tag mAb antibody (AE001) at a dilution of 1:1000.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AE001? Please let us know so that we can cite the reference in this datasheet.