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Mouse anti HA-Tag (N Terminal) mAb (AE065)

Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Western blot - Mouse anti HA-Tag (N Terminal) mAb (AE065)

Western blot analysis of lysates from 293T-SCRN1-HA-GFP-N, using Mouse anti HA-Tag mAb (AE065) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunoprecipitation - Mouse anti HA-Tag (N Terminal) mAb (AE065)

Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg Mouse anti HA-Tag antibody (AE065). Western blot was performed from the immunoprecipitate using Mouse anti HA-Tag antibody (AE065) at a dilution of 1:2000.

ABclonal:Immunoprecipitation - Mouse anti HA-Tag (N Terminal) mAb (AE065)

Immunoprecipitation analysis of 300ug extracts of 293F-SCRN1-HA-GFP(N) cells using 3ug Mouse anti HA-Tag mAb antibody (AE065 1:200). Western blot was performed from the immunoprecipitate using Mouse anti HA-Tag mAb antibody (AE065) at a dilition of 1:2000.

Overview

Product nameMouse anti HA-Tag (N Terminal) mAb
Catalog No.AE065
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG1, Kappa
CloneNo.AMC0173
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
ImmunogenA synthetic peptide corresponding to HA tag.
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsHA; HA tag; HA-tag
Calculated MW
Observed MW53kDa(SCRN1-HA-GFP-N)
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunoprecipitation    
Positive samples293T-SCRN1-HA-GFP-N
Cellular location
Customer validation

WB(Homo sapiens, Mus musculus)

IP(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Mouse anti HA-Tag (N Terminal) mAb (AE065)}

    Western blot - Mouse anti HA-Tag (N Terminal) mAb (AE065)

    Western blot analysis of lysates from 293T-SCRN1-HA-GFP-N, using Mouse anti HA-Tag mAb (AE065) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunoprecipitation - Mouse anti HA-Tag (N Terminal) mAb (AE065)}

    Immunoprecipitation - Mouse anti HA-Tag (N Terminal) mAb (AE065)

    Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg Mouse anti HA-Tag antibody (AE065). Western blot was performed from the immunoprecipitate using Mouse anti HA-Tag antibody (AE065) at a dilution of 1:2000.
    ABclonal:Immunoprecipitation - Mouse anti HA-Tag (N Terminal) mAb (AE065)}

    Immunoprecipitation - Mouse anti HA-Tag (N Terminal) mAb (AE065)

    Immunoprecipitation analysis of 300ug extracts of 293F-SCRN1-HA-GFP(N) cells using 3ug Mouse anti HA-Tag mAb antibody (AE065 1:200). Western blot was performed from the immunoprecipitate using Mouse anti HA-Tag mAb antibody (AE065) at a dilition of 1:2000.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AE065? Please let us know so that we can cite the reference in this datasheet.