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Mouse anti MYC-Tag mAb (AE038)

Publications (8) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Western blot - Mouse anti MYC-Tag mAb (AE038)

Western blot analysis of extracts of normal 293T cells, 293T transfected with NLK Protein and 293T transfected with YAP1 Protein, using Mouse anti MYC-Tag mAb (AE038) at 1:5000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product nameMouse anti MYC-Tag mAb
Catalog No.AE038
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG2b, Kappa
CloneNo.AMC0516
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
ImmunogenA synthetic peptide corresponding to Myc tag.
SequenceEQKLISEEDL
Gene ID
Swiss Prot
SynonymsMyc; Myc tag; Myc-tag
Calculated MW
Observed MW58kDa/72kDa
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:6000
  • IF/ICC 1:50 - 1:200
  • IP 1:50 - 1:100
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samples293T
Cellular location
Customer validation

WB(Oryza sativa, Homo sapiens, Arabidopsis thaliana)

(Homo sapiens)

IF(Homo sapiens)

IP(Homo sapiens)

Co-IP(Arabidopsis thaliana)

Co-IP(Oryza sativa)

Documents

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    ABclonal:Western blot - Mouse anti MYC-Tag mAb (AE038)}

    Western blot - Mouse anti MYC-Tag mAb (AE038)

    Western blot analysis of extracts of normal 293T cells, 293T transfected with NLK Protein and 293T transfected with YAP1 Protein, using Mouse anti MYC-Tag mAb (AE038) at 1:5000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AE038? Please let us know so that we can cite the reference in this datasheet.