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Mouse anti Myc-Tag mAb (AE010)

Review (1)Publications (112) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Mouse anti Myc-Tag mAb
ABclonal:Western blot - Mouse anti Myc-Tag mAb (AE010)

Western blot analysis of lysates from wild type (WT) and 293F cells transfected with NLK-Myc (C-terminal) or PTEN-Myc (C-terminal) using Mouse anti Myc-Tag mAb (AE010) at 1:10000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020)
.Exposure time: 30s.

ABclonal:Immunofluorescence - Mouse anti Myc-Tag mAb (AE010)

Confocal imaging of 293F cells transfected with SQSTM1-Myc(N) cells using Mouse anti Myc-Tag mAb (AE010, dilution 1:2000) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunoprecipitation - Mouse anti Myc-Tag mAb (AE010)

Immunoprecipitation of NLK-Myc from 300 µg extracts of 293F cells transfected with a NLK expression vector containing a single C-terminal Myc-Tag was performed using 3 µg of Mouse anti Myc-Tag mAb (AE010). Mouse IgG isotype control (AC011) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10 % of the total input. Western blot analysis of immunoprecipitates was conducted using Mouse anti Myc-Tag mAb (AE102) at a dilution of 1:3000.

Review
ABclonal: review for Mouse anti Myc-Tag mAb(AE010)

Overview

Product nameMouse anti Myc-Tag mAb
Catalog No.AE010
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG1
CloneNo.AMC0504
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
ImmunogenA synthetic peptide corresponding to Myc tag.
SequenceEQKLISEEDL
Gene ID
Swiss Prot
SynonymsMyc; Myc tag; Myc-tag
Calculated MW
Observed MW62kDa, 60kDa
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:6000
  • IF 1:1000-1:5000
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunoprecipitation    
Positive samples293F transfected with NLK-Myc (C-terminal), 293F transfected with PTEN-Myc (C-terminal)
Cellular location
Customer validation

(HepG2)

WB(Homo sapiens, Mus musculus, Arabidopsis thaliana, Oryza sativa, Other, Solanum tuberosum, Zea mays, Spodoptera exigua, Medicago truncatula, Sus scrofa domesticus, Glycine max, Saccharomyces cerevisiae, Ictalurus punctatus, Takifugu rubripes, Triticum aestivum, Danio rerio, Gallus gallus)

IF(Homo sapiens, Arabidopsis thaliana)

IP(Mus musculus, Helicoverpa armigera, N. benthamiana, Homo sapiens, Lactobacillus vitis)

IF(Homo sapiens, Mus musculus)

ChIP(Arabidopsis thaliana)

Co-IP(Homo sapiens, Arabidopsis thaliana, Other, Oryza sativa, Xenopus laevis, Mus musculus, Rattus norvegicus)

Co-IP(Homo sapiens, Mus musculus, Oryza sativa)

IP(Homo sapiens, Mus musculus, Saccharomyces cerevisiae, Ictalurus punctatus, Other)

ChIP(Arabidopsis thaliana)

WB(Homo sapiens, Arabidopsis thaliana, Other)

IF(Homo sapiens,Other)

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    ABclonal:Western blot - Mouse anti Myc-Tag mAb (AE010)}

    Western blot - Mouse anti Myc-Tag mAb (AE010)

    Western blot analysis of lysates from wild type (WT) and 293F cells transfected with NLK-Myc (C-terminal) or PTEN-Myc (C-terminal) using Mouse anti Myc-Tag mAb (AE010) at 1:10000 dilution incubated overnight at 4℃.
    Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
    Lysates/proteins: 30 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020)
    .Exposure time: 30s.
    ABclonal:Immunofluorescence - Mouse anti Myc-Tag mAb (AE010)}

    Immunofluorescence - Mouse anti Myc-Tag mAb (AE010)

    Confocal imaging of 293F cells transfected with SQSTM1-Myc(N) cells using Mouse anti Myc-Tag mAb (AE010, dilution 1:2000) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
    ABclonal:Immunoprecipitation - Mouse anti Myc-Tag mAb (AE010)}

    Immunoprecipitation - Mouse anti Myc-Tag mAb (AE010)

    Immunoprecipitation of NLK-Myc from 300 µg extracts of 293F cells transfected with a NLK expression vector containing a single C-terminal Myc-Tag was performed using 3 µg of Mouse anti Myc-Tag mAb (AE010). Mouse IgG isotype control (AC011) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10 % of the total input. Western blot analysis of immunoprecipitates was conducted using Mouse anti Myc-Tag mAb (AE102) at a dilution of 1:3000.

    * For research use only. Not for therapeutic or diagnostic purposes.

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