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Mouse anti RFP-Tag mAb (AE020)

Publications (13) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Western blot - Mouse anti RFP-Tag mAb (AE020)

Western blot analysis of recombinant RFP protein using Mouse anti RFP-Tag mAb (AE020) at dilution of 1:10000.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 10ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Overview

Product nameMouse anti RFP-Tag mAb
Catalog No.AE020
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG1
CloneNo.AMC0509
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
ImmunogenRecombinant protein of RFP-Tag
SequenceMASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGVATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHHLFL
Gene ID
Swiss Prot
SynonymsRFP; RFP tag; RFP-tag
Calculated MW27kDa
Observed MWRefer to Figures
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:5000-1:20000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesRecombinant RFP protein
Cellular location
Customer validation

WB(Helicoverpa armigera, Cotton bollworms, Nicotiana benthamiana, Oryza sativa, Homo sapiens, Saccharomyces cerevisiae)

IP(Gallus gallus)

ChIP(Homo sapiens)

Co-IP(Penaeus japonicus, Other, Helicoverpa armigera)

WB(Other)

Co-IP(Helicoverpa armigera)

    ABclonal:Western blot - Mouse anti RFP-Tag mAb (AE020)}

    Western blot - Mouse anti RFP-Tag mAb (AE020)

    Western blot analysis of recombinant RFP protein using Mouse anti RFP-Tag mAb (AE020) at dilution of 1:10000.
    Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
    Lysates/proteins: 10ng per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AE020? Please let us know so that we can cite the reference in this datasheet.