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Mouse anti Strep II-Tag mAb (AE066)

Publications (22) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Western blot - Mouse anti Strep II-Tag mAb (AE066)

Western blot analysis of over-expressed Strep II-tagged protein in 293T cells using Mouse anti Strep II-Tag mAb (AE066).Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.

Overview

Product nameMouse anti Strep II-Tag mAb
Catalog No.AE066
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG1, Kappa
CloneNo.AMC0521
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
ImmunogenA synthetic peptide corresponding to Strep II tag.
SequenceWSHPQFEK
Gene ID
Swiss Prot
SynonymsStrep II; Strep II tag; Strep II-tag
Calculated MW
Observed MW32kDa
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:5000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T
Cellular location
Customer validation

WB(Cytophaga hutchinsonii, Homo sapiens, Saccharomyces cerevisiae, Pseudomonas putida, Sus scrofa, Anatinae, Other, Mus musculus)

IF(Homo sapiens)

IP(Anatinae, Other)

Pull-down(Escherichia coli, Homo sapiens)

WB(Escherichia coli)

Other(Mus musculus)

Co-IP(Escherichia coli)

IF(Other)

    ABclonal:Western blot - Mouse anti Strep II-Tag mAb (AE066)}

    Western blot - Mouse anti Strep II-Tag mAb (AE066)

    Western blot analysis of over-expressed Strep II-tagged protein in 293T cells using Mouse anti Strep II-Tag mAb (AE066).Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AE066? Please let us know so that we can cite the reference in this datasheet.