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Mouse anti V5-Tag mAb (AE017)

Publications (15) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Western blot - Mouse anti V5-Tag mAb (AE017)

Western blot analysis of recombinant Mouse V5 protein using Mouse anti V5-Tag mAb (AE017) at dilution of 1:2000.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 10ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunoprecipitation - Mouse anti V5-Tag mAb (AE017)

Immunoprecipitation analysis of 300ug extracts of CHO cells using 3ug Mouse anti V5-Tag mAb antibody (AE017 1:50). Western blot was performed from the immunoprecipitate using Mouse anti V5-Tag mAb antibody (AE017) at a dilition of 1:2000.

Overview

Product nameMouse anti V5-Tag mAb
Catalog No.AE017
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG2b, Kappa
CloneNo.AMC0506
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
ImmunogenA synthetic peptide corresponding to V5 tag.
SequenceGKPIPNPLLGLDST
Gene ID
Swiss Prot
SynonymsV5; V5 tag; V5-tag
Calculated MW
Observed MWRefer to Figures
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samplesRecombinant Mouse V5 protein
Cellular location
Customer validation

WB(Homo sapiens, Escherichia coli, Mus musculus, Other)

IP(Homo sapiens, Mus musculus)

Co-IP(Homo sapiens)

IF(Mus musculus, Drosophila melanogaster)

RIP(Drosophila melanogaster)

ChIP(Drosophila melanogaster)

IP(Mus musculus)

ChIP(Drosophila melanogaster)

Co-IP(Drosophila melanogaster)

IP(Drosophila melanogaster)

WB(Drosophila melanogaster)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Mouse anti V5-Tag mAb (AE017)}

    Western blot - Mouse anti V5-Tag mAb (AE017)

    Western blot analysis of recombinant Mouse V5 protein using Mouse anti V5-Tag mAb (AE017) at dilution of 1:2000.
    Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
    Lysates/proteins: 10ng per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunoprecipitation - Mouse anti V5-Tag mAb (AE017)}

    Immunoprecipitation - Mouse anti V5-Tag mAb (AE017)

    Immunoprecipitation analysis of 300ug extracts of CHO cells using 3ug Mouse anti V5-Tag mAb antibody (AE017 1:50). Western blot was performed from the immunoprecipitate using Mouse anti V5-Tag mAb antibody (AE017) at a dilition of 1:2000.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AE017? Please let us know so that we can cite the reference in this datasheet.