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Mouse anti mCherry-Tag mAb (AE002)

Publications (28) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Western blot - Mouse anti mCherry-Tag mAb (AE002)

Western blot analysis of extracts of normal 293T cells and 293T transfected with CT55 Protein, using Mouse anti mCherry-Tag mAb (AE002) at 1:5000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product nameMouse anti mCherry-Tag mAb
Catalog No.AE002
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG1
CloneNo.AMC0502
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
ImmunogenRecombinant protein of mCherry tag
SequenceMLSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK
Gene ID
Swiss Prot
SynonymsmCherry; mCherry tag; mCherry-tag
Calculated MW
Observed MW55kDa
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:5000 - 1:10000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 1% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T-CT55-mcherry
Cellular location
Customer validation

WB(Homo sapiens, Danio rerio, Mus musculus, Cryptobranchidae, Felis catus, Other, Nicotiana benthamiana, Glycine max, Takifugu rubripes, Arabidopsis thaliana, Chlorocebus aethiops, Drosophila melanogaster)

IF(Mus musculus, Homo sapiens, Mus musculus, Danio rerio, Other)

IP(Homo sapiens)

IF(Mus musculus, Homo sapiens, Chlorocebus aethiops)

Injection(Mus musculus)

IP(Homo sapiens, Chlorocebus aethiops)

Documents

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    ABclonal:Western blot - Mouse anti mCherry-Tag mAb (AE002)}

    Western blot - Mouse anti mCherry-Tag mAb (AE002)

    Western blot analysis of extracts of normal 293T cells and 293T transfected with CT55 Protein, using Mouse anti mCherry-Tag mAb (AE002) at 1:5000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AE002? Please let us know so that we can cite the reference in this datasheet.