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N6-methyladenosine / m6A Rabbit mAb (A19841)

Publications (28) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Dot Blot - N6-methyladenosine / m6A Rabbit mAb (A19841)

The m6A rabbit monoclonal antibody (A19841) are tested in Dot Blot against N6-methyladenosine (m6A) and unmodified adenosine.
Oligomer 8 - ATAACTGG-m6A-CCGAATGG
Oligomer 9 - ATAACTGGACCGAATGG
Oligomer 10 - AAAAAAAAAAAAAAAA-biotin.

ABclonal:Immunofluorescence - N6-methyladenosine / m6A Rabbit mAb (A19841)

Immunofluorescence analysis of U-2 OS treated with UV(30s)+Brdu(0min) and U-2 OS treated with UV(30s)+Brdu(10min) cells using N6-methyladenosine / m6A Rabbit mAb (A19841) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:RNA Binding Protein Immunoprecipitation - N6-methyladenosine / m6A Rabbit mAb (A19841)

RNA Immunoprecipitation was performed on 100 µg mouse liver total RNA , using 5 µg of the N6-methyladenosine / m6A Rabbit mAb. An equal amount of IgG was used as negative control. The immunoprecipitated RNA was verified by using HS6ST1 as PCR primer of qRT-PCR . The picture shows the relative enrichment multiple of HS6ST1 site.

Overview

Product nameN6-methyladenosine / m6A Rabbit mAb
Catalog No.A19841
Host speciesRabbit
Purification methodProtein A
IsotypeIgG
CloneNo.ARC5003-10
Discovered in the 1970s, m6A is the most prevalent internal modification in polyadenylated mRNAs and long non-coding RNAs (lncRNAs) in higher eukaryotes. m6A is widely conserved among eukaryotic species that range from yeast, plants, flies to mammals, as well as among viral RNAs with a nuclear phase. The m6A-based modification is associated with a well-defined RNA motif, RRACH (R: A/G, H: A/C/U). As a representative of the epitranscriptome, m6A mRNA modifications participate in many vital activities in the cell, including stem cell self-renewal and differentiation, mRNA transcription, alternative splicing, nuclear export, translation, degradation, and microRNA processing. These processes determine the expression or inactivation of specific genes, which is vital for growth and development.(PMID: 30416848; PMID: 24662220; PMID: 30429466)
ImmunogenChemical compounds corresponding to N6-methyladenosine / m6A.
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsN6-methyladenosine; m6A; N6-methyladenosine / m6A
Calculated MW
Observed MW
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • DB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
  • meRIP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationImmunofluorescence    
Positive samplesArabidopsis
Cellular location
Customer validation

DB(Homo sapiens, Rattus norvegicus, Gossypium hirsutum)

RIP(Homo sapiens, Sus scrofa, Mus musculus)

Dot(Homo sapiens)

Co-IP(Homo sapiens)

IF(Homo sapiens)

WB(Mus musculus)

Other(Homo sapiens, Mus musculus)

qPCR(Homo sapiens)

IHC(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Dot Blot - N6-methyladenosine / m6A Rabbit mAb (A19841)}

    Dot Blot - N6-methyladenosine / m6A Rabbit mAb (A19841)

    The m6A rabbit monoclonal antibody (A19841) are tested in Dot Blot against N6-methyladenosine (m6A) and unmodified adenosine.
    Oligomer 8 - ATAACTGG-m6A-CCGAATGG
    Oligomer 9 - ATAACTGGACCGAATGG
    Oligomer 10 - AAAAAAAAAAAAAAAA-biotin.
    ABclonal:Immunofluorescence - N6-methyladenosine / m6A Rabbit mAb (A19841)}

    Immunofluorescence - N6-methyladenosine / m6A Rabbit mAb (A19841)

    Immunofluorescence analysis of U-2 OS treated with UV(30s)+Brdu(0min) and U-2 OS treated with UV(30s)+Brdu(10min) cells using N6-methyladenosine / m6A Rabbit mAb (A19841) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:RNA Binding Protein Immunoprecipitation - N6-methyladenosine / m6A Rabbit mAb (A19841)}

    RNA Binding Protein Immunoprecipitation - N6-methyladenosine / m6A Rabbit mAb (A19841)

    RNA Immunoprecipitation was performed on 100 µg mouse liver total RNA , using 5 µg of the N6-methyladenosine / m6A Rabbit mAb. An equal amount of IgG was used as negative control. The immunoprecipitated RNA was verified by using HS6ST1 as PCR primer of qRT-PCR . The picture shows the relative enrichment multiple of HS6ST1 site.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A19841? Please let us know so that we can cite the reference in this datasheet.