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N6-methyladenosine / m6A Rabbit pAb (A17924)

Publications (14) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Dot Blot - N6-methyladenosine / m6A Rabbit pAb (A17924)

The m6A rabbit polyclonal antibody (4µg, A17924) are tested in Nucleotide Array against N6-methyladenosine (m6A) and unmodified adenosine (100pmol for each oligomer).
Oligomer 1 - N6-methyladenosine (m6A-CUGGUAACGAAUGGCU-G-Biotin)
Oligomer 2 - unmodified adenosine (ACUGGUAACGAAUGGCU-G-Biotin)
Oligomer 3 - unmodified adenosine (AAAAAAAAAAAAAAAAA-Biotin)

ABclonal:Dot Blot - N6-methyladenosine / m6A Rabbit pAb (A17924)

The m6A rabbit polyclonal antibody (500ng/100ng, A17924) are tested in Dot Blot against total RNA of Arabidopsis.
(This image is courtesy of an anonymous Abreview)

ABclonal:Dot Blot - N6-methyladenosine / m6A Rabbit pAb (A17924)

The m6A rabbit polyclonal antibody (A17924) are tested in Dot Blot against N6-methyladenosine (m6A) and unmodified adenosine.
Oligomer 8 - ATAACTGG-m6A-CCGAATGG
Oligomer 9 - ATAACTGGACCGAATGG
Oligomer 10 - AAAAAAAAAAAAAAAA-biotin.

ABclonal:Immunofluorescence - N6-methyladenosine / m6A Rabbit pAb (A17924)

U2OS cells pre-treated with BrdU were subjected UVC irradiation incubated at 37 °C for the indicated time, and stained for m6A Polyclonal Antibody (A17924). DAPI, 4′, 6-diamidino-2-phenylindole. Global UVC irradiation exceed cytoplasmic leavel, peaking at 2 min after irradiation and diminishing over the following 8 min.

ABclonal:Immunoprecipitation - N6-methyladenosine / m6A Rabbit pAb (A17924)

Immunoprecipitation analysis of 25μg total RNA extracts of Arabidopsis (25μg total RNA extracts of Arabidopsis was diluted to 200 µl IP buffer system) using 2ul m6A antibody (A17924).

Overview

Product nameN6-methyladenosine / m6A Rabbit pAb
Catalog No.A17924
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Discovered in the 1970s, m6A is the most prevalent internal modification in polyadenylated mRNAs and long non-coding RNAs (lncRNAs) in higher eukaryotes. m6A is widely conserved among eukaryotic species that range from yeast, plants, flies to mammals, as well as among viral RNAs with a nuclear phase. The m6A-based modification is associated with a well-defined RNA motif, RRACH (R: A/G, H: A/C/U). As a representative of the epitranscriptome, m6A mRNA modifications participate in many vital activities in the cell, including stem cell self-renewal and differentiation, mRNA transcription, alternative splicing, nuclear export, translation, degradation, and microRNA processing. These processes determine the expression or inactivation of specific genes, which is vital for growth and development.(PMID: 30416848; PMID: 24662220; PMID: 30429466)
ImmunogenChemical compounds corresponding to N6-methyladenosine / m6A.
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsN6-methyladenosine; m6A; N6-methyladenosine / m6A
Calculated MW
Observed MW
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • DB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
  • meRIP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationImmunofluorescence    
Positive samplesArabidopsis
Cellular location
Customer validation

RIP(Homo sapiens, Mus musculus)

PCR(Homo sapiens)

IP(Homo sapiens, Mus musculus)

DB(Homo sapiens)

IF(Mus musculus)

WB(Mus musculus)

IHC(Mus musculus)

Other(Homo sapiens,Mus musculus)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Dot Blot - N6-methyladenosine / m6A Rabbit pAb (A17924)}

    Dot Blot - N6-methyladenosine / m6A Rabbit pAb (A17924)

    The m6A rabbit polyclonal antibody (4µg, A17924) are tested in Nucleotide Array against N6-methyladenosine (m6A) and unmodified adenosine (100pmol for each oligomer).
    Oligomer 1 - N6-methyladenosine (m6A-CUGGUAACGAAUGGCU-G-Biotin)
    Oligomer 2 - unmodified adenosine (ACUGGUAACGAAUGGCU-G-Biotin)
    Oligomer 3 - unmodified adenosine (AAAAAAAAAAAAAAAAA-Biotin)
    ABclonal:Dot Blot - N6-methyladenosine / m6A Rabbit pAb (A17924)}

    Dot Blot - N6-methyladenosine / m6A Rabbit pAb (A17924)

    The m6A rabbit polyclonal antibody (500ng/100ng, A17924) are tested in Dot Blot against total RNA of Arabidopsis.
    (This image is courtesy of an anonymous Abreview)
    ABclonal:Dot Blot - N6-methyladenosine / m6A Rabbit pAb (A17924)}

    Dot Blot - N6-methyladenosine / m6A Rabbit pAb (A17924)

    The m6A rabbit polyclonal antibody (A17924) are tested in Dot Blot against N6-methyladenosine (m6A) and unmodified adenosine.
    Oligomer 8 - ATAACTGG-m6A-CCGAATGG
    Oligomer 9 - ATAACTGGACCGAATGG
    Oligomer 10 - AAAAAAAAAAAAAAAA-biotin.
    ABclonal:Immunofluorescence - N6-methyladenosine / m6A Rabbit pAb (A17924)}

    Immunofluorescence - N6-methyladenosine / m6A Rabbit pAb (A17924)

    U2OS cells pre-treated with BrdU were subjected UVC irradiation incubated at 37 °C for the indicated time, and stained for m6A Polyclonal Antibody (A17924). DAPI, 4′, 6-diamidino-2-phenylindole. Global UVC irradiation exceed cytoplasmic leavel, peaking at 2 min after irradiation and diminishing over the following 8 min.
    ABclonal:Immunoprecipitation - N6-methyladenosine / m6A Rabbit pAb (A17924)}

    Immunoprecipitation - N6-methyladenosine / m6A Rabbit pAb (A17924)

    Immunoprecipitation analysis of 25μg total RNA extracts of Arabidopsis (25μg total RNA extracts of Arabidopsis was diluted to 200 µl IP buffer system) using 2ul m6A antibody (A17924).

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A17924? Please let us know so that we can cite the reference in this datasheet.