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Nitro-Tyrosine Rabbit pAb (A20506)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Nitro-Tyrosine Rabbit pAb (A20506)

Western blot analysis of various lysates using Nitro-Tyrosine Rabbit pAb (A20506) at 1:1000 dilution.HeLa and C6 cells were treated by Perxynitrate（3mM）for 1 hours.NIH3T3 cells were treated by Perxynitrate（3mM）for 3 hours.Jurkat cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product nameNitro-Tyrosine Rabbit pAb
Catalog No.A20506
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Tyrosine nitration of proteins is one of the most important oxidative post-translational modifications in vivo. A major obstacle for its biochemical and physiological studies is the lack of efficient and chemoselective protein tyrosine nitration reagents.
ImmunogenChemical compounds corresponding to Nitro-Tyrosine.
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW10-250kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa treated by Perxynitrate, NIH/3T3 treated by Perxynitrate, C6 treated by Perxynitrate
Cellular location

Documents

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    ABclonal:Western blot - Nitro-Tyrosine Rabbit pAb (A20506)}

    Western blot - Nitro-Tyrosine Rabbit pAb (A20506)

    Western blot analysis of various lysates using Nitro-Tyrosine Rabbit pAb (A20506) at 1:1000 dilution.HeLa and C6 cells were treated by Perxynitrate（3mM）for 1 hours.NIH3T3 cells were treated by Perxynitrate（3mM）for 3 hours.Jurkat cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.