제품 > 항체 > 다중클론항체(pAb)

OsWRKY30 Rabbit pAb (A20659)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Oryza sativa

ABclonal:Western blot - OsWRKY30 Rabbit pAb (A20659)

Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using OsWRKY30 Rabbit pAb (A20659) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 120s.

Overview

Product nameOsWRKY30 Rabbit pAb
Catalog No.A20659
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
OsWRKY30 functions as a positive regulator of rice disease resistance via an SA signaling pathway.
ImmunogenRecombinant protein of Oryza sativa OsWRKY30.
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsOsWRKY30
Calculated MW24kDa
Observed MW30kDa
ReactivityOryza sativa
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesSpikes
Cellular location
    ABclonal:Western blot - OsWRKY30 Rabbit pAb (A20659)}

    Western blot - OsWRKY30 Rabbit pAb (A20659)

    Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using OsWRKY30 Rabbit pAb (A20659) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 120s.

    * For research use only. Not for therapeutic or diagnostic purposes.