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PE Mouse anti DDDDK-Tag mAb (C-terminal) (AE116)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Flow CytoMetry - PE Mouse anti DDDDK-Tag mAb (C-terminal) (AE116)

Flow cytometry: 1X10^6 CHO cells (negative control) and CHO(Transfection, right) cells were intracellularly-stained with PE Mouse anti DDDDK-Tag mAb(AE116, 5 μl/Test, orange line) or PE Mouse IgG isotype control (5 μl/Test, blue line).Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - PE Mouse anti DDDDK-Tag mAb (C-terminal) (AE116)

Flow cytometry:1X10^6 CHO(Transfection) cells were intracellularly-stained with PE Mouse IgG isotype control (5 μl/Test, left) or PE Mouse anti DDDDK-Tag mAb(AE116, 5 μl/Test, right).

Overview

Product namePE Mouse anti DDDDK-Tag mAb (C-terminal)
Catalog No.AE116
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG1, Kappa
CloneNo.AMC0382-PE
FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography.A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis and Western blotting.
ImmunogenA synthetic peptide corresponding to DDDDK tag.
SequenceDYKDDDDK
Gene ID
Swiss Prot
SynonymsDDDDK; DDDDK tag; DDDDK-tag
Calculated MW
Observed MW
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC (intra) 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.03% proclin300, pH7.3.
Key application
Positive samples
Cellular location
Customer validation

FC(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Flow CytoMetry - PE Mouse anti DDDDK-Tag mAb (C-terminal) (AE116)}

    Flow CytoMetry - PE Mouse anti DDDDK-Tag mAb (C-terminal) (AE116)

    Flow cytometry: 1X10^6 CHO cells (negative control) and CHO(Transfection, right) cells were intracellularly-stained with PE Mouse anti DDDDK-Tag mAb(AE116, 5 μl/Test, orange line) or PE Mouse IgG isotype control (5 μl/Test, blue line).Non-fluorescently stained cells were used as blank control (red line).
    ABclonal:Flow CytoMetry - PE Mouse anti DDDDK-Tag mAb (C-terminal) (AE116)}

    Flow CytoMetry - PE Mouse anti DDDDK-Tag mAb (C-terminal) (AE116)

    Flow cytometry:1X10^6 CHO(Transfection) cells were intracellularly-stained with PE Mouse IgG isotype control (5 μl/Test, left) or PE Mouse anti DDDDK-Tag mAb(AE116, 5 μl/Test, right).

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AE116? Please let us know so that we can cite the reference in this datasheet.