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PE Mouse anti-Rabbit IgG, light chain specific mAb (AS129)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity: Rabbit

ABclonal:Flow CytoMetry - PE Mouse anti-Rabbit IgG,  light chain specific mAb (AS129)

Flow cytometry: 1X10^6 BeWo cells (negative control, left) and HeLa cells (right) were surface-stained with DR4 Rabbit mAb (A24703, 2 μg/mL, orange line) or Rabbit IgG isotype control (AC042, 2 μg/mL, blue line), followed by PE Mouse anti-Rabbit IgG, light chain specific Ab (AS129, 5 μl/Test) staining. Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - PE Mouse anti-Rabbit IgG,  light chain specific mAb (AS129)

Flow cytometry: 1X10^6 HeLa cells were surface-stained with Rabbit IgG isotype control (AC042, 2 μg/mL, left) or DR4 Rabbit mAb (A24703, 2 μg/mL, right). PE Mouse anti-Rabbit IgG, light chain specific Ab (AS129, 5 μl/Test) was used as a secondary antibody.

Overview

Product namePE Mouse anti-Rabbit IgG, light chain specific mAb
Catalog No.AS129
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG2b, kappa
CloneNo.AMC50031-PE
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
ImmunogenRecombinant protein of rabbit IgG.
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW
Reactivity Rabbit
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.09% Sodium azide, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular location
    ABclonal:Flow CytoMetry - PE Mouse anti-Rabbit IgG,  light chain specific mAb (AS129)}

    Flow CytoMetry - PE Mouse anti-Rabbit IgG, light chain specific mAb (AS129)

    Flow cytometry: 1X10^6 BeWo cells (negative control, left) and HeLa cells (right) were surface-stained with DR4 Rabbit mAb (A24703, 2 μg/mL, orange line) or Rabbit IgG isotype control (AC042, 2 μg/mL, blue line), followed by PE Mouse anti-Rabbit IgG, light chain specific Ab (AS129, 5 μl/Test) staining. Non-fluorescently stained cells were used as blank control (red line).
    ABclonal:Flow CytoMetry - PE Mouse anti-Rabbit IgG,  light chain specific mAb (AS129)}

    Flow CytoMetry - PE Mouse anti-Rabbit IgG, light chain specific mAb (AS129)

    Flow cytometry: 1X10^6 HeLa cells were surface-stained with Rabbit IgG isotype control (AC042, 2 μg/mL, left) or DR4 Rabbit mAb (A24703, 2 μg/mL, right). PE Mouse anti-Rabbit IgG, light chain specific Ab (AS129, 5 μl/Test) was used as a secondary antibody.

    * For research use only. Not for therapeutic or diagnostic purposes.