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PE Rabbit anti-DDDDK-Tag mAb (A27398)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Flow CytoMetry - PE Rabbit anti-DDDDK-Tag mAb (A27398)

Flow cytometry: 1X10^6 293T cells (negative control, left) and 293T (Transfection, right) cells were surface-stained with PE Rabbit anti-DDDDK-Tag mAb (A27398, 5 μl/Test, orange line) or PE Rabbit IgG isotype control (A24172, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - PE Rabbit anti-DDDDK-Tag mAb (A27398)

Flow cytometry: 1X10^6 293T (Transfection) cells were surface-stained with PE Rabbit IgG isotype control (A24172, 5 μl/Test, left) or PE Rabbit anti-DDDDK-Tag mAb (A27398, 5 μl/Test, right).

ABclonal:Flow CytoMetry - PE Rabbit anti-DDDDK-Tag mAb (A27398)

Flow cytometry: 1X10^6 293T cells (negative control, left) and 293T (Transfection, right) cells were intracellularly-stained with PE Rabbit anti-DDDDK-Tag mAb (A27398, 5 μl/Test, orange line) or PE Rabbit IgG isotype control (A24172, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - PE Rabbit anti-DDDDK-Tag mAb (A27398)

Flow cytometry: 1X10^6 293T (Transfection) cells were intracellularly-stained with PE Rabbit IgG isotype control (A24172, 5 μl/Test, left) or PE Rabbit anti-DDDDK-Tag mAb (A27398, 5 μl/Test, right).

Overview

Product namePE Rabbit anti-DDDDK-Tag mAb
Catalog No.A27398
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC73185-PE
FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography.A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis and Western blotting.
ImmunogenA synthetic peptide corresponding to DDDDK tag.
SequenceDYKDDDDK
Gene ID
Swiss Prot
SynonymsDDDDK; DDDDK tag; DDDDK-tag
Calculated MW
Observed MW
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC (intra) 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key application
Positive samples
Cellular location

Documents

Certificate of Compliance

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Lot number

    ABclonal:Flow CytoMetry - PE Rabbit anti-DDDDK-Tag mAb (A27398)}

    Flow CytoMetry - PE Rabbit anti-DDDDK-Tag mAb (A27398)

    Flow cytometry: 1X10^6 293T cells (negative control, left) and 293T (Transfection, right) cells were surface-stained with PE Rabbit anti-DDDDK-Tag mAb (A27398, 5 μl/Test, orange line) or PE Rabbit IgG isotype control (A24172, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
    ABclonal:Flow CytoMetry - PE Rabbit anti-DDDDK-Tag mAb (A27398)}

    Flow CytoMetry - PE Rabbit anti-DDDDK-Tag mAb (A27398)

    Flow cytometry: 1X10^6 293T (Transfection) cells were surface-stained with PE Rabbit IgG isotype control (A24172, 5 μl/Test, left) or PE Rabbit anti-DDDDK-Tag mAb (A27398, 5 μl/Test, right).
    ABclonal:Flow CytoMetry - PE Rabbit anti-DDDDK-Tag mAb (A27398)}

    Flow CytoMetry - PE Rabbit anti-DDDDK-Tag mAb (A27398)

    Flow cytometry: 1X10^6 293T cells (negative control, left) and 293T (Transfection, right) cells were intracellularly-stained with PE Rabbit anti-DDDDK-Tag mAb (A27398, 5 μl/Test, orange line) or PE Rabbit IgG isotype control (A24172, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
    ABclonal:Flow CytoMetry - PE Rabbit anti-DDDDK-Tag mAb (A27398)}

    Flow CytoMetry - PE Rabbit anti-DDDDK-Tag mAb (A27398)

    Flow cytometry: 1X10^6 293T (Transfection) cells were intracellularly-stained with PE Rabbit IgG isotype control (A24172, 5 μl/Test, left) or PE Rabbit anti-DDDDK-Tag mAb (A27398, 5 μl/Test, right).

    * For research use only. Not for therapeutic or diagnostic purposes.