제품 > 항체 > 단일클론항체(mAb)

PE Rabbit anti-Mouse IgG1 mAb (A25697)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Flow CytoMetry - PE Rabbit anti-Mouse IgG1 mAb (A25697)

Flow cytometry: 1X10^6 PC-3 cells (negative control, left) and THP-1 cells (right) were surface-stained with Mouse anti-Human CD4 mAb (IgG1, κ, orange line) or Mouse IgG1 isotype control (2 μg/mL, blue line), followed by PE Rabbit anti-Mouse IgG1 mAb (A25697, 5 μl/Test) staining. Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - PE Rabbit anti-Mouse IgG1 mAb (A25697)

Flow cytometry: 1X10^6 THP-1 cells were surface-stained with Mouse IgG1 isotype control (2 μg/mL, left) or Mouse anti-Human CD4 mAb (IgG1, κ, right). PE Rabbit anti-Mouse IgG1 mAb (A25697, 5 μl/Test) was used as a secondary antibody.

Overview

Product namePE Rabbit anti-Mouse IgG1 mAb
Catalog No.A25697
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC63967-PE
Mouse IgG1 is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG1 is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG1 is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 98-324 of mouse IgG1(P01868).
SequenceAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSPRPSETVTCNVAHPASSTKVDKKIVPRDCGCKPCICTVPEVSSVFIFPPKPKDVLTITLTPKVTCVVVDISKDDPEVQFSWFVDDVEVHTAQTQPREEQFNSTFRSVSELPIMHQDWLNGKEFKCRVNSAAFPAPIEKTISKTKGRPKAPQVYTIPPPKEQMAKDKVSLTCMITDFFPEDITVEWQWNGQPAENYKNTQPIMNTNGSYFVYSKLNVQKSNWEAGNTFTCSVLHEGLHNHHTEKSLSHSPGK
Gene ID
Swiss Prot
SynonymsIgG1
Calculated MW36kDa
Observed MW
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationSecreted.

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Flow CytoMetry - PE Rabbit anti-Mouse IgG1 mAb (A25697)}

    Flow CytoMetry - PE Rabbit anti-Mouse IgG1 mAb (A25697)

    Flow cytometry: 1X10^6 PC-3 cells (negative control, left) and THP-1 cells (right) were surface-stained with Mouse anti-Human CD4 mAb (IgG1, κ, orange line) or Mouse IgG1 isotype control (2 μg/mL, blue line), followed by PE Rabbit anti-Mouse IgG1 mAb (A25697, 5 μl/Test) staining. Non-fluorescently stained cells were used as blank control (red line).
    ABclonal:Flow CytoMetry - PE Rabbit anti-Mouse IgG1 mAb (A25697)}

    Flow CytoMetry - PE Rabbit anti-Mouse IgG1 mAb (A25697)

    Flow cytometry: 1X10^6 THP-1 cells were surface-stained with Mouse IgG1 isotype control (2 μg/mL, left) or Mouse anti-Human CD4 mAb (IgG1, κ, right). PE Rabbit anti-Mouse IgG1 mAb (A25697, 5 μl/Test) was used as a secondary antibody.

    * For research use only. Not for therapeutic or diagnostic purposes.