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Pan DiMethyl-lysine Rabbit pAb (A18296)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Pan DiMethyl-lysine Rabbit pAb (A18296)

Western blot analysis of various lysates using Pan DiMethyl-lysine Rabbit pAb (A18296) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

Overview

Product namePan DiMethyl-lysine Rabbit pAb
Catalog No.A18296
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The lysine methylation chemical reaction is the reversible addition of one, two or three methyl groups to the ε-nitrogen of a lysine side chain, forming monomethylated, dimethylated and trimethylated derivatives (referred to here as ‘Kme1’, ‘Kme2’ and ‘Kme3’).
ImmunogenA synthetic peptide corresponding to a sequence containing dimethylated K.
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW17-48kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesNIH/3T3, C6, Mouse liver, Rat liver, HeLa, MCF7
Cellular location
Customer validation

WB(C. albicans)

Documents

Certificate of Compliance

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    ABclonal:Western blot - Pan DiMethyl-lysine Rabbit pAb (A18296)}

    Western blot - Pan DiMethyl-lysine Rabbit pAb (A18296)

    Western blot analysis of various lysates using Pan DiMethyl-lysine Rabbit pAb (A18296) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 3min.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A18296? Please let us know so that we can cite the reference in this datasheet.